Transcript: Human XM_024449315.1

PREDICTED: Homo sapiens WASP family member 3 (WASF3), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
WASF3 (10810)
Length:
4799
CDS:
208..1716

Additional Resources:

NCBI RefSeq record:
XM_024449315.1
NBCI Gene record:
WASF3 (10810)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024449315.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000381495 GCATCGGACGTTACGGATTAC pLKO_005 931 CDS 100% 10.800 15.120 N WASF3 n/a
2 TRCN0000122939 GCCTACTACATTGGCGCTATT pLKO.1 3625 3UTR 100% 10.800 15.120 N WASF3 n/a
3 TRCN0000353047 GCCTACTACATTGGCGCTATT pLKO_005 3625 3UTR 100% 10.800 15.120 N WASF3 n/a
4 TRCN0000122941 CCAGCGCAGATAATTGAGTAT pLKO.1 1198 CDS 100% 4.950 6.930 N WASF3 n/a
5 TRCN0000344129 CCAGCGCAGATAATTGAGTAT pLKO_005 1198 CDS 100% 4.950 6.930 N WASF3 n/a
6 TRCN0000122942 CGCTGCTATTCGAATGGGAAT pLKO.1 1542 CDS 100% 4.050 5.670 N WASF3 n/a
7 TRCN0000344184 CGCTGCTATTCGAATGGGAAT pLKO_005 1542 CDS 100% 4.050 5.670 N WASF3 n/a
8 TRCN0000382089 CTAATCCTGTTGCTGATATTT pLKO_005 560 CDS 100% 15.000 10.500 N WASF3 n/a
9 TRCN0000380123 CTTCTACATCAGAGCAAATTC pLKO_005 390 CDS 100% 13.200 9.240 N Wasf3 n/a
10 TRCN0000122943 GCAAACATGCTGAAGACATAT pLKO.1 341 CDS 100% 13.200 9.240 N WASF3 n/a
11 TRCN0000122940 CCAGCGAACTTGAATGTGTAA pLKO.1 275 CDS 100% 4.950 3.465 N WASF3 n/a
12 TRCN0000353046 CCAGCGAACTTGAATGTGTAA pLKO_005 275 CDS 100% 4.950 3.465 N WASF3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024449315.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11555 pDONR223 100% 92.1% 90.6% None (many diffs) n/a
2 ccsbBroad304_11555 pLX_304 0% 92.1% 90.6% V5 (many diffs) n/a
3 TRCN0000475984 CGGTTCTGAACGAAAAGGTCGATA pLX_317 18.3% 92.1% 90.6% V5 (many diffs) n/a
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