Construct: ORF TRCN0000476011
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011949.1_s317c1
- Derived from:
- ccsbBroadEn_13556
- DNA Barcode:
- CCGCTTAAACATTCTTTATGGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SREK1IP1 (285672)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476011
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285672 | SREK1IP1 | SREK1 interacting protein 1 | NM_173829.4 | 64.1% | 52.2% | 235_236insA;300_465del |
| 2 | human | 285672 | SREK1IP1 | SREK1 interacting protein 1 | XM_011543344.3 | 53.8% | 41.5% | 13_14ins48;187_188insA;252_417del |
| 3 | mouse | 67288 | Srek1ip1 | splicing regulatory glutami... | NM_026075.3 | 55.9% | 50.9% | (many diffs) |
| 4 | mouse | 67288 | Srek1ip1 | splicing regulatory glutami... | NR_135272.1 | 13.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 89
- ORF end:
- 389
- ORF length:
- 300
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctTGCA ACAAATTGAT GAGCAATGCT TTTTTATAAT 61 GCCAACTTTG TACAAAAAAG TTGGCACCAT GGCAGTCCCA GGTTGCAACA AGGACAGTGT 121 CAGAGCAGGC TGTAAAAAAT GTGGCTACCC TGGTCACCTG ACTTTTGAAT GCCGCAATTT 181 TCTCCGAGTA GACCCTAAAA GGGACATAGT TTTGGATGTC AGCAGTACAA GTAGTGAAGA 241 TAGCGATGAA GAGAATGAAG AACTGAATAA ATTGCAGGCA TTACAGGAAA AAAGAATAAA 301 TGAAGAAGAG GAAAAGAAGA AAGAAAAAAA GCAAAGAAAA AATCAAATTG AAAAAAAAAA 361 GGAAAAGGTC TTACTCATCC AGTTCCACTT GCCAACTTTC TTGTACAAAG TGGTTGATAT 421 CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC 481 GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCGCTT 541 AAACATTCTT TATGGCCCAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt 601 gaaagatt