Construct: ORF TRCN0000476042
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007971.1_s317c1
- Derived from:
- ccsbBroadEn_09486
- DNA Barcode:
- CAAAGTAGTTGGATGCTTCACATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LSM12 (124801)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476042
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 124801 | LSM12 | LSM12 homolog | NM_001371445.1 | 99.8% | 99.4% | 230C>G |
| 2 | human | 124801 | LSM12 | LSM12 homolog | NM_152344.4 | 99.8% | 99.4% | 230C>G |
| 3 | human | 124801 | LSM12 | LSM12 homolog | NM_001369485.1 | 85.7% | 85.4% | 230C>G;369_464del |
| 4 | human | 124801 | LSM12 | LSM12 homolog | NM_001369484.1 | 78.5% | 69.6% | (many diffs) |
| 5 | human | 124801 | LSM12 | LSM12 homolog | NM_001369486.1 | 70.2% | 70.2% | 82_83ins174 |
| 6 | human | 124801 | LSM12 | LSM12 homolog | XM_011524309.3 | 54.1% | 46.6% | (many diffs) |
| 7 | mouse | 268490 | Lsm12 | LSM12 homolog | NM_172947.3 | 96.9% | 99.4% | (many diffs) |
| 8 | mouse | 268490 | Lsm12 | LSM12 homolog | XM_006533414.3 | 96.9% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 654
- ORF length:
- 585
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggctcct ccgggcgagt acttcagcgt tgggagccag gtgtcgtgcc 121 ggacgtgcca ggagcagcgg ctgcagggcg aggtggtagc ctttgactac caatccaaaa 181 tgctggcttt aaaatgtccc tcttccagtg gaaagcccaa ccatgcagac atcttgctca 241 taaacttaca gtatgtttca gaagtggaaa taattaatga ccgaacagaa acccctcgtc 301 ccctagcttc actcaatgtt agtaagcttg ccagcaaagc acggacagag aaggaggaga 361 agctgagcca ggcctatgca atcagtgctg gtgtctctct agagggccag cagctcttcc 421 agaccattca caagaccatt aaagactgta aatggcaaga aaaaaacatc GTAGTCATGG 481 AAGAAGTTGT TATTACACCC CCATATCAAG TGGAAAACTG TAAAGGCAAA GAGGGGAGTG 541 CACTGAGCCA TGTACGCAAA ATAGTTGAAA AACATTTTAG AGACGTGGAA AGCCAAAAGA 601 TACTGCAACG TTCACAAGCC CAGCAACCAC AGAAGGAGGC TGCCCTGTCA TCCTTGCCAA 661 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 721 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 781 TATCTTGTGG AAAGGACGAC AAAGTAGTTG GATGCTTCAC ATGACGCGTT AAGTCgacaa 841 tcaacctctg gattacaaaa tttgtgaaag att