Construct: ORF TRCN0000476066
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016894.1_s317c1
- Derived from:
- ccsbBroadEn_00235
- DNA Barcode:
- ATACCCCCAGCCGATTAGTACTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRIT1 (889)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476066
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350672.1 | 100% | 100% | |
| 2 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350673.1 | 100% | 100% | |
| 3 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350674.1 | 100% | 100% | |
| 4 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350675.1 | 100% | 100% | |
| 5 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350676.1 | 100% | 100% | |
| 6 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350677.1 | 100% | 100% | |
| 7 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350678.1 | 100% | 100% | |
| 8 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350679.1 | 100% | 100% | |
| 9 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350680.1 | 100% | 100% | |
| 10 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350681.1 | 100% | 100% | |
| 11 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350682.1 | 100% | 100% | |
| 12 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350683.1 | 100% | 100% | |
| 13 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350684.1 | 100% | 100% | |
| 14 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350685.1 | 100% | 100% | |
| 15 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350686.1 | 100% | 100% | |
| 16 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350687.1 | 100% | 100% | |
| 17 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350688.1 | 100% | 100% | |
| 18 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350689.1 | 100% | 100% | |
| 19 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350690.1 | 100% | 100% | |
| 20 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350691.1 | 100% | 100% | |
| 21 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350692.1 | 100% | 100% | |
| 22 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350693.1 | 100% | 100% | |
| 23 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350694.1 | 100% | 100% | |
| 24 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350695.1 | 100% | 100% | |
| 25 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350696.1 | 100% | 100% | |
| 26 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350697.1 | 100% | 100% | |
| 27 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_004912.4 | 100% | 100% | |
| 28 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_194454.2 | 100% | 100% | |
| 29 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_194455.1 | 100% | 100% | |
| 30 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_194456.1 | 100% | 100% | |
| 31 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001013406.2 | 93.4% | 93.4% | 845_846ins144 |
| 32 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350669.1 | 93.4% | 93.4% | 845_846ins144 |
| 33 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350670.1 | 93.4% | 93.4% | 845_846ins144 |
| 34 | human | 889 | KRIT1 | KRIT1 ankyrin repeat contai... | NM_001350671.1 | 67.4% | 67.1% | (many diffs) |
| 35 | mouse | 79264 | Krit1 | KRIT1, ankyrin repeat conta... | NM_030675.3 | 90.1% | 94.5% | (many diffs) |
| 36 | mouse | 79264 | Krit1 | KRIT1, ankyrin repeat conta... | XM_006503617.3 | 90.1% | 94.5% | (many diffs) |
| 37 | mouse | 79264 | Krit1 | KRIT1, ankyrin repeat conta... | XM_006503618.3 | 90.1% | 94.5% | (many diffs) |
| 38 | mouse | 79264 | Krit1 | KRIT1, ankyrin repeat conta... | NM_001170552.1 | 85.4% | 89.8% | (many diffs) |
| 39 | mouse | 79264 | Krit1 | KRIT1, ankyrin repeat conta... | NR_033173.1 | 31.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2277
- ORF length:
- 2208
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaaatcca gaaaacatag aagatgcata tgttgctgtt attcgtccaa 121 agaatactgc cagtctcaat tctcgggaat acagagctaa gtcatatgaa attttgttgc 181 atgaagttcc cattgaagga cagaaaaaaa agagaaagaa agttttattg gaaacgaaac 241 ttcaaggcaa cagtgaaata acacaaggca tattggatta cgtagtagaa accaccaaac 301 caatttctcc tgcaaaccag ggtatcagag gaaaacgagt tgtactaatg aaaaaatttc 361 ctctggatgg agagaagatg ggcagagaag catcattatt tattgttcca tcagttgtca 421 aagataatac taaatacaca tataccccag gatgcccaat tttttactgc ttacaagata 481 ttatgcgagt ctgtagtgaa tccagtactc attttgctac acttacagca aggatgttaa 541 tagccttgga taagtggtta gatgaacgtc atgcacaatc tcactttatt ccagctttat 601 tccgaccttc tcctcttgag cggataaaaa ctaatgtcat aaatcctgca tatgctactg 661 aatcaggtca gacagaaaac tcactacata tgggctatag tgcactagaa ataaagagta 721 aaatgttagc cctagagaaa gcagatacct gtatttacaa ccctttgttt ggatcagatc 781 ttcagtatac aaatcgggta gataaagtgg taataaatcc atactttggt ctaggagctc 841 cagactactc aaaaatccaa atacctaaac aggaaaaatg gcagagaagc atgagcagtg 901 tcacagaaga caaggaacga cagtgggtag atgattttcc tctccaccga agcgcctgtg 961 aaggagattc agaattacta agccgtcttc tcagtgaaag attttcagtc aaccagttag 1021 atagtgacca ctgggcaccc attcattatg catgctggta tggaaaagtt gaggccactc 1081 gcatattgtt agagaaagga aagtgcaatc caaacctttt aaatggacaa cttagttctc 1141 ctcttcattt tgctgctgga ggaggacatg ctgaaatagt acagattctc ctaaaccacc 1201 cagaaacgga tagacatata acagaccaac aaggaagatc tccattaaat atttgtgaag 1261 aaaacaaaca aaacaactgg gaagaagctg caaaattgtt gaaggaagca attaacaaac 1321 catatgaaaa agttcgaata tacagaatgg atgggtcata tcgttctgtt gaattgaagc 1381 atggaaataa taccacagtg cagcagataa tggaaggaat gcgtctctct caagaaactc 1441 agcaatattt cactatatgg atttgttcag aaaacctcag ccttcaactc aaaccatatc 1501 ataaaccctt gcaacatgtt cgtgactggc cagaaatact tgctgaattg actaatctgg 1561 atcctcaaag ggaaacacct cagctttttc taagaagaga tgtgagactt cccttggaag 1621 ttgaaaaaca gattgaagac ccactagcta ttcttattct ctttgatgaa gccagatata 1681 atttattgaa gggcttttat acagctcctg atgctaagct gataacattg gcaagtctgc 1741 ttttgcaaat agtctatgga aattatgaga gtaaaaaaca caagcaaggt ttcctaaatg 1801 aagaaaatct aaaatccatc gtacctgtta ccaaactgaa aagtaaggca cctcactgga 1861 caaatcgcat acttcatgaa tacaagaatc tcagtacaag tgaaggtgtc agtaaagaaa 1921 tgcaTCACCT TCAGCGCATG TTCTTACAGA ATTGCTGGGA AATTCCTACT TATGGAGCAG 1981 CATTTTTCAC AGGACAGATA TTTACAAAGG CAAGCCCCAG CAATCATAAA GTCATCCCTG 2041 TGTATGTAGG AGTGAATATA AAAGGACTTC ATCTCCTCAA CATGGAAACT AAGGCTTTAC 2101 TCATCAGTCT TAAGTATGGT TGTTTTATGT GGCAATTGGG AGATACTGAT ACTTGTTTTC 2161 AGATCCATAG CATGGAAAAT AAAATGAGCT TTATAGTACA TACAAAACAG GCTGGTCTCG 2221 TGGTAAAACT GTTAATGAAG CTAAATGGAC AGTTAATGCC CACTGAAAGA AATTCATTGC 2281 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2341 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2401 ATATATCTTG TGGAAAGGAC GAATACCCCC AGCCGATTAG TACTCTACGC GTTAAGTCga 2461 caatcaacct ctggattaca aaatttgtga aagatt