Construct: ORF TRCN0000476070
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014843.1_s317c1
- Derived from:
- ccsbBroadEn_10847
- DNA Barcode:
- AAGGCTTCATCAGAGTCATGCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- B4GALT1 (2683)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476070
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2683 | B4GALT1 | beta-1,4-galactosyltransfer... | XM_005251440.5 | 62.5% | 60.7% | (many diffs) |
| 2 | human | 2683 | B4GALT1 | beta-1,4-galactosyltransfer... | NM_001497.3 | 56.1% | 54.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 744
- ORF length:
- 675
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggcttcgg gagccgctcc tgagcggcag cgccgcgatg ccaggcgcgt 121 ccctacagcg ggcctgccgc ctgctcgtgg ccgtctgcgc tctgcacctt ggcgtcaccc 181 tcgtttacta cctggctggc cgcgacctga gccgcctgcc ccaactggtc ggagtctcca 241 caccgctgca gggcggctcg aacagtgccg ccgccatcgg gcagtcctcc ggggagctcc 301 ggaccggagg ggcccggccg ccgcctcctc taggcgcctc ctcccagccg cgcccgggtg 361 gcgactccag cccagtcgtg gattctggcc ctggccccgc tagcaacttg acctcggtcc 421 cagtgcccca caccaccgca ctgtcgctgc ccgcctgccc TGAGGAGTCC CCGCTGCTTG 481 TGGGCCCCAT GCTGATTGAG TTTAACATGC CTGTGGACCT GGAGCTCGTG GCAAAGCAGA 541 ACCCAAATGT GAAGATGGGC GGCCGCTATG CCCCCAGGGA CTGCGTCTCT CCTCACAAGG 601 TGGCCATCAT CATTCCATTC CGCAACCGGC AGGAGCACCT CAAGTACTGG CTATATTATT 661 TGCATCCAGT CCTGCAGCGC CAGCAGCTGG ACTATGGCAT CTATGTTATC AACCAGTATG 721 AAAAAATAAG AAGACTCCTG TGGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 781 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 841 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA AGGCTTCATC 901 AGAGTCATGC ACGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 961 att