Construct: ORF TRCN0000476081
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006163.1_s317c1
- Derived from:
- ccsbBroadEn_11726
- DNA Barcode:
- ATATAGACGACCTATTACGTTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM189A1 (23359)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476081
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23359 | FAM189A1 | family with sequence simila... | NM_015307.1 | 52.3% | 35.1% | (many diffs) |
| 2 | human | 23359 | FAM189A1 | family with sequence simila... | XM_011521407.2 | 48.9% | 32.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 918
- ORF length:
- 849
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgctgtatg cagatggtct cctccgatgt cctgcagatg ttccttccgc 121 agaggtcaca tcctgccaac cccacctgcg tgactcctca cggcaccgtt ctccaccaga 181 ccctggattt cgacgagttc atccccccac tcccgcctcc accctattac cccccagagt 241 acacctgcac accgtccact gaggcccaga ggggcctcca cctggacttt gctccgtctc 301 cattcggcac tttgtatgac gtggccatca atagccccgg cctgctctat cctgctgagc 361 tcccccctcc gtacgaggcg gtggtgggcc agccccctgc cagccaggtt acaagtatag 421 gtcagcaggt ggccgagtcc agctccgggg acccaaacac cagtgctggc ttcagcactc 481 cagtaccagc tgacagcaca agcctcctgg tgtccgaggg cactgctacg ccaggttcca 541 gcccatcccc cgatggccct gtgggcgccc cagcacccTC CGAACCTGCC CTTCCCCTGG 601 CCGCGTGTCT CCAGAGGATC CTGGCATGGG CTCACAGGTG CAGCCAGGTC CCGGGCATGT 661 GTCCCGCTCC ACCAGCGACC CCACCTTGTG CACATCTAGC ATGGCAGGCG ATGCCTCTTC 721 ACACAGGCCG TCGTGTAGCC AGGACCTGGA AGCGGGACTG TCTGAGGCTG TGCCTGGGAG 781 CGCTTCCATG TCTCGCTCTG CCACGGCTGC CTGTCGGGCC CAGCTTTCAC CAGCGGGGGA 841 CCCAGATACC TGGAAAACTG ACCAACGGCC AACACCAGAG CCCTTCCCAG CGACCTCCAA 901 AGAGCGGCCA CGCTCTTTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 961 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1021 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAATATAGA CGACCTATTA 1081 CGTTTACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt