Construct: ORF TRCN0000476087
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003459.1_s317c1
- Derived from:
- ccsbBroadEn_09100
- DNA Barcode:
- TTTCGACCTCCCATAAAGGGCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPR101 (83550)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476087
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83550 | GPR101 | G protein-coupled receptor 101 | NM_054021.1 | 99.8% | 99.8% | 667C>T;1350C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1590
- ORF length:
- 1524
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gtccacctgc accaacagca cgcgcgagag taacagcagc cacacgtgca 121 tgcccctctc caaaatgccc atcagcctgg cccacggcat catccgctca accgtgctgg 181 ttatcttcct cgccgcctct ttcgtcggca acatagtgct ggcgctagtg ttgcagcgca 241 agccgcagct gctgcaggtg accaaccgtt ttatctttaa cctcctcgtc accgacctgc 301 tgcagatttc gctcgtggcc ccctgggtgg tggccacctc tgtgcctctc ttctggcccc 361 tcaacagcca cttctgcacg gccctggtta gcctcaccca cctgttcgcc ttcgccagcg 421 tcaacaccat tgtcgtggtg tcagtggatc gctacttgtc catcatccac cctctctcct 481 acccgtccaa gatgacccag cgccgcggtt acctgctcct ctatggcacc tggattgtgg 541 ccatcctgca gagcactcct ccactctacg gctggggcca ggctgccttt gatgagcgca 601 atgctctctg ctccatgatc tggggggcca gccccagcta cactattctc agcgtggtgt 661 ccttcatcgt cattccactg attgtcatga ttgcctgcta ctccgtggtg ttctgtgcag 721 cccggaggca gtatgctctg ctgtacaatg tcaagagaca cagcttggaa gtgcgagtca 781 aggactgtgt ggagaatgag gatgaagagg gagcagagaa gaaggaggag ttccaggatg 841 agagtgagtt tcgccgccag catgaaggtg aggtcaaggc caaggagggc agaatggaag 901 ccaaggacgg cagcctgaag gccaaggaag gaagcacggg gaccagtgag agtagtgtag 961 aggccagggg cagcgaggag gtcagagaga gcagcacggt ggccagcgac ggcagcatgg 1021 agggtaagga aggcagcacc aaagttgagg agaacagcat gaaggcagac aagggtcgca 1081 cagaggtcaa ccagtgcagc attgacttgg gtgaagatga catggagttt ggtgaagacg 1141 acatcaattt cagtgaggat gacgtcgagg cagtgaacat cccggagagc ctcccaccca 1201 gtcgtcgtaa cagcaacagc aaccctccTC TGCCCAGGTG CTACCAGTGC AAAGCTGCTA 1261 AAGTGATCTT CATCATCATT TTCTCCTATG TGCTATCCCT GGGGCCCTAC TGCTTTTTAG 1321 CAGTCCTGGC CGTGTGGGTG GATGTCGAAA CCCAGGTACC CCAGTGGGTG ATCACCATAA 1381 TCATCTGGCT TTTCTTCCTG CAGTGCTGCA TCCATCCCTA TGTCTATGGC TACATGCACA 1441 AGACCATTAA GAAGGAAATC CAGGACATGC TGAAGAAGTT CTTCTGCAAG GAAAAGCCCC 1501 CGAAAGAAGA TAGCCACCCA GACCTGCCCG GAACAGAGGG TGGGACTGAA GGCAAGATTG 1561 TCCCTTCCTA CGATTCTGCT ACTTTTCCTT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1621 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1681 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTTCG 1741 ACCTCCCATA AAGGGCGCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1801 tgaaagatt