Construct: ORF TRCN0000476107
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008690.1_s317c1
- Derived from:
- ccsbBroadEn_10586
- DNA Barcode:
- ACACTAGTTGTGTTTCCCCCATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSUN5P2 (260294)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476107
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | XR_242255.5 | 55.3% | (many diffs) | |
| 2 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_001168347.3 | 54.9% | 47.3% | (many diffs) |
| 3 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_148956.4 | 54% | 47.3% | (many diffs) |
| 4 | human | 260294 | NSUN5P2 | NSUN5 pseudogene 2 | NR_033323.3 | 52.8% | (many diffs) | |
| 5 | human | 155400 | NSUN5P1 | NSUN5 pseudogene 1 | NR_033322.3 | 51.9% | (many diffs) | |
| 6 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_001168348.2 | 50.9% | 46.2% | (many diffs) |
| 7 | human | 155400 | NSUN5P1 | NSUN5 pseudogene 1 | NR_104013.1 | 49% | (many diffs) | |
| 8 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | NM_018044.4 | 47% | 42.8% | (many diffs) |
| 9 | human | 55695 | NSUN5 | NOP2/Sun RNA methyltransfer... | XM_005277604.5 | 38% | 18.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1014
- ORF length:
- 945
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctggagctg ctggtgtttc ccgcccagac agatctgcat gaacacccac 121 tgtaccgggc cggacacctc attctgcagg acagggccag ctgtctccca gccatgctgc 181 tggacccccg ccaggctccc atgtcatgga tgcctgtgcc accccaggca ataaagacca 241 gtcacttggc tgctcttctg aagaaccaag ggaagatctt tgcctttgac ctggatgcca 301 ggcggctggc atccatggcc acgctgctgg cctgggctgg cgtctcctgc tgtgagctgg 361 ctgaggagga cttcctggcg gtctccccct tagatccgcg ctatcgtgag gtccactatg 421 tcctgctgga tccttcctgc agtggctcgg gtatgccgag cagacagctg gaggagcccg 481 gggcagggac acctagcccg gtgcgtctgc atgccctggc agggttccag cagcgagccc 541 tgtgccacgc gctcactttc ccttccctgc agcggctcgt ctactccatg tgctccctct 601 gccaggagga gaatgaagac atggtacaag atgcgctgca gcagaacccg ggcgccTTCA 661 GGCTAGCTCC CGCCCTGCCT GCCCGGCCCC ACCGAGGCCT GAGCACGTTC CCGGGTGCCG 721 AGCACTGCCT CCGGGCTTCC CCCAAGACCA CGCTTAGCGG TGGCTTCTTC GTTGCTGTAA 781 TTGAACGGGT CGAGATGCCG ACCTCAGCCT CACAGGCCAA AGCATCAGCA CCAGAACGCA 841 CACCCAGCCC AGCCCCAAAG AGAAAGAAGA GAGCAAAAAG CTGCAGCCGG TGCTTGCACA 901 CCGCCTTGCA CATAGCAGAG GCTCCGGGCT CACTCCTTCC TGGTGGAAAA GGAAGATGCC 961 TGTCCTCTCC GTGGAAGACC CTGGGCCCTC ACCGCAGGCA GCAGTTTGCG TTTTTGCCAA 1021 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1081 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1141 TATCTTGTGG AAAGGACGAA CACTAGTTGT GTTTCCCCCA TAAACGCGTT AAGTCgacaa 1201 tcaacctctg gattacaaaa tttgtgaaag att