Construct: ORF TRCN0000476116
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010256.1_s317c1
- Derived from:
- ccsbBroadEn_04463
- DNA Barcode:
- ATCGCATCATGACAAATTGGAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIGY (84992)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476116
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84992 | PIGY | phosphatidylinositol glycan... | NM_001042616.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 306
- ORF length:
- 213
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGTTTCT GTCTCTTCCT ACGTTGACTG 121 TTCTTATTCC ACTGGTTTCT TTAGCAGGAC TGTTCTACTC AGCCTCTGTG GAAGAAAACT 181 TCCCACAGGG CTGCACTAGC ACAGCCAGCC TTTGCTTTTA CAGCCTGCTC TTGCCTATTA 241 CCATACCAGT GTATGTATTC TTCCACCTTT GGACTTGGAT GGGTATTAAA CTCTTCAGGC 301 ATAATTTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 361 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 421 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATCGCATCA TGACAAATTG GAATCACGCG 481 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt