Construct: ORF TRCN0000476146
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000861.1_s317c1
- Derived from:
- ccsbBroadEn_07894
- DNA Barcode:
- GTAACACGGTGTGCGGCATGCGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTREX (23517)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476146
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23517 | MTREX | Mtr4 exosome RNA helicase | NM_015360.5 | 99.9% | 99.9% | 2698C>A;2916C>A |
| 2 | mouse | 72198 | Skiv2l2 | superkiller viralicidic act... | NM_028151.2 | 88.2% | 97.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3195
- ORF length:
- 3126
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggacgca ttcggagatg agctgttcag cgtgttcgag ggcgactcga 121 ccactgcggc gggaaccaaa aaagacaagg aaaaggacaa ggggaaatgg aaggggcctc 181 cagggtctgc agacaaggca gggaaacgtt ttgatggtaa attacaatca gaatcaacta 241 ataatggaaa aaataagaga gatgtagatt tcgaaggtac agatgaaccc atttttggaa 301 agaagcccag gatagaagag tcaataactg aagacttaag tttggcagac ctgatgccca 361 gagtcaaggt acaatcagtt gaaactgttg aagggtgtac acatgaggtt gcacttcctg 421 cagaagagga ttatttacca cttaaaccac gagttggaaa agctgctaag gaatacccgt 481 tcattcttga tgcttttcaa agagaggcca ttcagtgtgt tgacaataat cagtctgttc 541 tagtatctgc acatacctca gcgggaaaaa cagtatgcgc cgagtatgcc attgcattgg 601 ccttaaggga aaagcagcgt gtaatattta ccagcccaat taaggctctg agtaaccaaa 661 aataccgtga aatgtatgaa gaatttcaag atgttggttt gatgactggt gatgttacta 721 ttaatcctac ggcatcttgt cttgttatga ccacagagat tttgagaagt atgctttaca 781 gaggttccga agttatgaga gaagttgctt gggttatatt tgatgaaatt cattatatga 841 gagattcaga acgtggtgta gtatgggaag aaactattat tttgcttcct gataacgtcc 901 actatgtctt tctttcggct actattccaa atgcccgaca gtttgctgaa tggatttgcc 961 atttacataa acagccttgt catgttattt acacagatta tcggcccact ccattgcaac 1021 actacatttt tccagcaggg ggagatggcc tgcatcttgt ggttgatgaa aatggtgact 1081 tcagagaaga taattttaat actgcaatgc aagtgcttcg agatgcaggt gatttggcca 1141 aaggagacca gaaagggcgg aaaggaggaa caaaaggacc atcaaatgtt ttcaaaattg 1201 tgaagatgat tatggaaaga aatttccaac ctgtgattat tttcagtttt agtaagaaag 1261 attgtgaagc ctatgcactt caaatgacca aattagattt caacacagat gaagaaaaga 1321 agatggttga agaagtattc agtaatgcaa ttgattgctt atccgatgaa gataaaaaac 1381 tccctcaggt agaacatgta cttcctcttt tgaagagggg aattggtatt caccatggtg 1441 gtttacttcc tattttgaaa gaaactatag aaattctctt ttctgaagga ttgataaagg 1501 ccttatttgc cacggagacc tttgctatgg gaattaacat gccagctaga actgttttat 1561 ttacaaatgc ccgcaaattt gatgggaagg atttccgatg gatttcttct ggtgaataca 1621 ttcagatgtc tggtcgtgct ggaaggagag gaatggatga tagaggaatt gtaattctta 1681 tggtagatga aaagatgagc ccaacaattg gaaaacaatt acttaagggc tccgctgatc 1741 ctctaaatag tgctttccat ttgacctaca acatggtttt gaacttacta cgtgtagaag 1801 aaattaatcc tgagtacatg ttggaaaaat ccttctacca gtttcagcat tatagagcaa 1861 ttccaggagt agtagagaag gtaaagaatt cagaagaaca gtataataaa atagtaattc 1921 ccaatgaaga aagtgtggtt atctattata agattagaca gcagcttgcc aaattgggta 1981 aagaaattga agaatatatt cacaaaccaa aatactgctt accttttcta caaccaggtc 2041 gtttggtaaa ggtaaagaat gaaggagatg actttggctg gggagtagtg gtgaatttct 2101 caaaaaagtc aaatgttaag cctaactctg gtgaactgga tcctttgtat gtagtagaag 2161 tacttctgcg ctgtagcaaa gagagcttga aaaattcagc tacagaagct gcaaaaccag 2221 ctaaacctga tgagaaagga gagatgcagg ttgtcccagt tttggtgcat ctcctgtctg 2281 ctatcagcag tgttaggctt tacattccta aagaccttcg gccggtggac aatagacaga 2341 gtgttttaaa atcaatacag gaagttcaga aacgttttcc tgacggcatc cccttattag 2401 accctattga tgatatgggc attcaagatc aagggctgaa aaaagtcatt cagaaagtag 2461 aagcttttga gcatcgaatg tattctcatc cacttcacaa tgatccaaat ttggaaactg 2521 tgtatacgct ttgtgaaaaa aaagcacaga ttgcaataga tattaaatct gcaaagcgag 2581 aactgaagaa agcaagaaca gtcctacaaa tggatgaact caaatgtcgc aaacgtgttt 2641 taagaaggtt gggatttgct acttcttctg atgtaataga gatgaaagga cgagtggctt 2701 gtgagataag cagtgctgat gagctccttc taactgagat gatgtttaat ggccttttca 2761 atgacatttc TGCAGAACAG GCAACAGCAT TATTAAGCTG CTTTGTGTTT CAAGAGAATT 2821 CTAGTGAGAT GCCCAAATTA ACAGAACAAT TAGCAGGACC ACTTCGTCAA ATGCAGGAAT 2881 GTGCTAAAAG AATTGCAAAA GTTTCAGCAG AAGCCAAATT GGAAATTGAT GAGGAAACTT 2941 ATCTAAGCTC ATTTAAACCT CACTTAATGG ATGTAGTATA TACATGGGCA ACTGGAGCTA 3001 CATTTGCCCA TATCTGCAAA ATGACAGATG TCTTTGAAGG CAGCATAATT CGTTGTATGA 3061 GGCGCCTGGA AGAATTGCTT CGACAAATGT GTCAAGCAGC AAAAGCCATT GGAAACACTG 3121 AGCTGGAAAA TAAATTTGCA GAAGGAATCA CCAAAATCAA GAGAGATATT GTGTTTGCTG 3181 CCAGCCTCTA CTTGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 3241 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 3301 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTAACACGGT GTGCGGCATG 3361 CGCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt