Construct: ORF TRCN0000476147
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005110.1_s317c1
- Derived from:
- ccsbBroadEn_05817
- DNA Barcode:
- CCCAATGTTAGAGCAAGGAATAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANPEP (290)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476147
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 290 | ANPEP | alanyl aminopeptidase, memb... | NM_001150.3 | 99.9% | 100% | 2136C>A;2178C>A |
| 2 | human | 290 | ANPEP | alanyl aminopeptidase, memb... | XM_005254892.4 | 99.9% | 100% | 2136C>A;2178C>A |
| 3 | human | 290 | ANPEP | alanyl aminopeptidase, memb... | XM_011521473.1 | 99.9% | 100% | 2136C>A;2178C>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2967
- ORF length:
- 2901
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caagggcttc tatatttcca agtccctggg catcctgggg atcctcctgg 121 gcgtggcagc cgtgtgcaca atcatcgcac tgtcagtggt gtactcccag gagaagaaca 181 agaacgccaa cagctccccc gtggcctcca ccaccccgtc cgcctcagcc accaccaacc 241 ccgcctcggc caccaccttg gaccaaagta aagcgtggaa tcgttaccgc ctccccaaca 301 cgctgaaacc cgattcctac cgggtgacgc tgagaccgta cctcaccccc aatgacaggg 361 gcctgtacgt ttttaagggc tccagcaccg tccgtttcac ctgcaaggag gccactgacg 421 tcatcatcat ccacagcaag aagctcaact acaccctcag ccaggggcac agggtggtcc 481 tgcgtggtgt gggaggctcc cagccccccg acattgacaa gactgagctg gtggagccca 541 ccgagtacct ggtggtgcac ctcaagggct ccctggtgaa ggacagccag tatgagatgg 601 acagcgagtt cgagggggag ttggcagatg acctggcggg cttctaccgc agcgagtaca 661 tggagggcaa tgtcagaaag gtggtggcca ctacacagat gcaggctgca gatgcccgga 721 agtccttccc atgcttcgat gagccggcca tgaaggccga gttcaacatc acgcttatcc 781 accccaagga cctgacagcc ctgtccaaca tgcttcccaa aggtcccagc accccacttc 841 cagaagaccc caactggaat gtcactgagt tccacaccac gcccaagatg tccacgtact 901 tgctggcctt cattgtcagt gagttcgact acgtggagaa gcaggcatcc aatggtgtct 961 tgatccggat ctgggcccgg cccagtgcca ttgcggcggg ccacggcgat tatgccctga 1021 acgtgacggg ccccatcctt aacttctttg ctggtcatta tgacacaccc tacccactcc 1081 caaaatcaga ccagattggc ctgccagact tcaacgccgg cgccatggag aactggggac 1141 tggtgaccta ccgggagaac tccctgctgt tcgaccccct gtcctcctcc agcagcaaca 1201 aggagcgggt ggtcactgtg attgctcatg agctggccca ccagtggttc gggaacctgg 1261 tgaccataga gtggtggaat gacctgtggc tgaacgaggg cttcgcctcc tacgtggagt 1321 acctgggtgc tgactatgcg gagcccacct ggaacttgaa agacctcatg gtgctgaatg 1381 atgtgtaccg cgtgatggca gtggatgcac tggcctcctc ccacccgctg tccacacccg 1441 cctcggagat caacacgccg gcccagatca gtgagctgtt tgacgccatc tcctacagca 1501 agggcgcctc agtcctcagg atgctctcca gcttcctgtc cgaggacgta ttcaagcagg 1561 gcctggcgtc ctacctccac acctttgcct accagaacac catctacctg aacctgtggg 1621 accacctgca ggaggctgtg aacaaccggt ccatccaact ccccaccacc gtgcgggaca 1681 tcatgaaccg ctggaccctg cagatgggct tcccggtcat cacggtggat accagcacgg 1741 ggaccctttc ccaggagcac ttcctccttg accccgattc caatgttacc cgcccctcag 1801 aattcaacta cgtgtggatt gtgcccatca catccatcag agatggcaga cagcagcagg 1861 actactggct gatagatgta agagcccaga acgatctctt cagcacatca ggcaatgagt 1921 gggtcctgct gaacctcaat gtgacgggct attaccgggt gaactacgac gaagagaact 1981 ggaggaagat tcagactcag ctgcagagag accactcggc catccctgtc atcaatcggg 2041 cacagatcat taatgacgcc ttcaacctgg ccagtgccca taaggtccct gtcactctgg 2101 cgctgaacaa caccctcttc ctgattgaag agagacagta catgccctgg gaggccgccc 2161 tgagcagcct gagctacttc aagctcatgt ttgaccgctc agaggtctat ggccccatga 2221 agaactacct gaagaagcag gtaacacccc tcttcattca cttcagaaat aataccaaca 2281 actggaggga gatcccagaa aacctgatgg accagtacag cgaggttaat gccatcagca 2341 ccgcctgctc caacggagtt ccagagtgtg aggagatggt ctctggcctt ttcaagcagt 2401 ggatggagaa ccccaataat aacccgatcc accccaacct gcggtccacc gtctactgca 2461 acgctatcgc ccagggcggg gaggaggagt gggacttcgc ctgggagcag ttccgaaatg 2521 ccacactggt caatgaggct gacaagctcc gggcagccct ggcctgcagc aaagagttgt 2581 ggatcctgaa caggtaccTG AGCTACACCC TGAACCCGGA CTTAATCCGG AAGCAGGACG 2641 CCACCTCTAC CATCATCAGC ATTACCAACA ACGTCATTGG GCAAGGTCTG GTCTGGGACT 2701 TTGTCCAGAG CAACTGGAAG AAGCTTTTTA ACGATTATGG TGGTGGCTCG TTCTCCTTCT 2761 CCAACCTCAT CCAGGCAGTG ACACGACGAT TCTCCACCGA GTATGAGCTG CAGCAGCTGG 2821 AGCAGTTCAA GAAGGACAAC GAGGAAACAG GCTTCGGCTC AGGCACCCGG GCCCTGGAGC 2881 AAGCCCTGGA GAAGACGAAA GCCAACATCA AGTGGGTGAA GGAGAACAAG GAGGTGGTGC 2941 TCCAGTGGTT CACAGAAAAC AGCAAATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 3001 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3061 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCCAATGT 3121 TAGAGCAAGG AATAGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3181 aagatt