Construct: ORF TRCN0000476154
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003534.1_s317c1
- Derived from:
- ccsbBroadEn_00619
- DNA Barcode:
- CCAGTAATGAATTTCAATAAAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GBX2 (2637)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476154
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2637 | GBX2 | gastrulation brain homeobox 2 | NM_001485.4 | 100% | 100% | |
| 2 | human | 2637 | GBX2 | gastrulation brain homeobox 2 | NM_001301687.2 | 58.1% | 50% | 523_559del;666_667ins415 |
| 3 | mouse | 14472 | Gbx2 | gastrulation brain homeobox 2 | NM_010262.3 | 92% | 98.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1113
- ORF length:
- 1044
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcgcagcg ttcccgccgt cgctgatgat gatgcagcgc ccgctgggga 121 gtagcaccgc cttcagcata gactcgctga tcggcagccc gccgcagccc agccccggcc 181 atttcgtcta caccggctac cccatgttca tgccctaccg gccggtagtg ctgccgccgc 241 cgccgccgcc gccgcccgcg ctgccccagg ccgcgctgca gccagcgctg ccgcccgcac 301 accctcacca ccagatcccc agcctgccca caggcttctg ctccagcctg gcgcagggca 361 tggcgctcac ctctacgctc atggccacgc tccccggcgg cttctccgcg tcgccccagc 421 accaggaggc ggcagcggcc cgcaagttcg cgccgcagcc gctgcccggc ggcggtaact 481 tcgacaaggc ggaggcgctg caggctgacg cggaggacgg caaaggcttc ctggccaaag 541 agggctcgct gctcgccttc tccgcggccg agacggtgca ggcttcgctc gtcggggctg 601 tccgagggca agggaaagac gagtcaaagg tggaagacga cccgaagggc aaggaggaga 661 gcttctcgct ggagagcgat gtggactaca gctcggatga caatctgact ggccaggcag 721 ctcacaagga ggaagacccg ggccacgcgc tggaggagac cccgccgagc agcggcgccg 781 cgggcagcac cacgtcTACG GGCAAGAACC GGCGGCGGCG GACTGCCTTC ACCAGCGAGC 841 AGCTGCTGGA GCTAGAGAAG GAGTTCCACT GCAAAAAGTA CCTCTCCTTG ACCGAGCGCT 901 CGCAGATCGC CCACGCCCTC AAACTCAGCG AGGTGCAGGT GAAAATCTGG TTCCAGAACC 961 GACGGGCCAA GTGGAAACGG GTGAAGGCAG GCAATGCCAA TTCCAAGACA GGGGAGCCCT 1021 CCCGGAACCC TAAGATCGTC GTCCCCATCC CTGTCCACGT CAGCAGGTTC GCTATCAGAA 1081 GTCAGCATCA GCAGCTAGAA CAGGCCCGGC CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1141 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1201 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 1261 AGTAATGAAT TTCAATAAAA TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1321 ttgtgaaaga tt