Transcript: Human NM_001301687.2

Homo sapiens gastrulation brain homeobox 2 (GBX2), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
GBX2 (2637)
Length:
2177
CDS:
416..1084

Additional Resources:

NCBI RefSeq record:
NM_001301687.2
NBCI Gene record:
GBX2 (2637)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001301687.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000433110 ACGTCAGCAGGTTCGCTATCA pLKO_005 1441 3UTR 100% 4.950 6.930 N GBX2 n/a
2 TRCN0000020442 CCATTTCGTCTACACCGGCTA pLKO.1 526 CDS 100% 2.160 3.024 N GBX2 n/a
3 TRCN0000020443 CACCACGTCTACGGGCAAGAA pLKO.1 1172 3UTR 100% 1.650 1.320 N GBX2 n/a
4 TRCN0000020440 GCAAGGGAAAGACGAGTCAAA pLKO.1 992 CDS 100% 4.950 3.465 N GBX2 n/a
5 TRCN0000020441 CGCTATCAGAAGTCAGCATCA pLKO.1 1454 3UTR 100% 4.050 2.835 N GBX2 n/a
6 TRCN0000412456 CACCGCCTTCAGCATAGACTC pLKO_005 472 CDS 100% 1.350 0.945 N GBX2 n/a
7 TRCN0000020439 CCACTGTGTTTGAAGCAACAA pLKO.1 1571 3UTR 100% 4.950 2.970 N GBX2 n/a
8 TRCN0000415051 TGTGGACTACAGCTCGGATGA pLKO_005 1064 CDS 100% 4.050 2.430 N GBX2 n/a
9 TRCN0000070657 CGGACTGCCTTCACCAGCGAA pLKO.1 1203 3UTR 100% 0.000 0.000 N Gbx2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001301687.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00619 pDONR223 100% 58.1% 50% None 523_559del;666_667ins415 n/a
2 ccsbBroad304_00619 pLX_304 0% 58.1% 50% V5 523_559del;666_667ins415 n/a
3 TRCN0000476154 CCAGTAATGAATTTCAATAAAATC pLX_317 30.6% 58.1% 50% V5 523_559del;666_667ins415 n/a
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