Construct: ORF TRCN0000476173
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008246.1_s317c1
- Derived from:
- ccsbBroadEn_05088
- DNA Barcode:
- GCTCCAGGTTCCCAACCGATCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AMZ1 (155185)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476173
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 155185 | AMZ1 | archaelysin family metallop... | NM_133463.3 | 99.9% | 100% | 1137G>A |
| 2 | human | 155185 | AMZ1 | archaelysin family metallop... | XM_011515150.2 | 99.9% | 100% | 1137G>A |
| 3 | human | 155185 | AMZ1 | archaelysin family metallop... | NM_001321766.1 | 65.1% | 64.4% | (many diffs) |
| 4 | human | 155185 | AMZ1 | archaelysin family metallop... | XM_011515151.3 | 59.7% | 45% | (many diffs) |
| 5 | human | 155185 | AMZ1 | archaelysin family metallop... | XM_017011774.2 | 59.7% | 45% | (many diffs) |
| 6 | human | 155185 | AMZ1 | archaelysin family metallop... | NM_001284355.3 | 59.6% | 44.6% | 600_601ins170;891_892ins433 |
| 7 | human | 155185 | AMZ1 | archaelysin family metallop... | XM_017011776.1 | 54.4% | 52.8% | (many diffs) |
| 8 | human | 155185 | AMZ1 | archaelysin family metallop... | XM_017011775.2 | 54% | 41.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1563
- ORF length:
- 1494
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgcagtgt agacccgcac aggagttcag cttcgggccc cgggccttga 121 aggacgctct ggtctccact gacgcagccc tgcagcagct gtatgtgtcc gccttctccc 181 ctgccgagcg gctcttcctg gccgaggcct acaacccgca gaggacgctc ttctgcaccc 241 tgctcatccg cacgggcttc gactggctcc tgagccgacc cgaggctccc gaggacttcc 301 agaccttcca cgcctccctg cagcaccgga agccccgcct ggctcggaag cacatctacc 361 tacagccgat agacctgagc gaggagccgg tgggaagctc cctgctgcac cagctgtgca 421 gctgcacaga ggccttcttc ctgggcctgc gcgtcaagtg cctgccgtcg gtggcagccg 481 cgtccatccg ctgctcctcg cggcccagcc gggactctga caggctccag ctccacacag 541 acggcatcct gtccttcttg aagaacaaca agccagggga cgcgctgtgt gtgctgggcc 601 tcacactgtc tgacctgtac ccccatgagg cctggagctt caccttcagc aagttccttc 661 cagggcacga agtgggcgtc tgcagcttcg cccggttctc aggggaattc ccgaagtcgg 721 ggcccagcgc ccctgatctg gccctggtag aggcagcagc agacggcccc gaggcccccc 781 tgcaggacag gggctgggcc ctgtgcttca gtgccctggg gatggttcag tgctgcaagg 841 tcacgtgcca cgagctctgc caccttctgg gcctggggaa ctgccgctgg ctccgctgcc 901 tcatgcaggg tgcgctcagc ctggacgagg ccctgcggcg gcccctggac ctctgtccca 961 tctgcctgag gaagctgcag catgtcctgg gtttcaggct catcgagagg taccagagac 1021 tctacacctg gactcaggcg gtggtgggga cgtggcccag ccaggaggcg ggggagccgt 1081 cagtgtggga ggacaccccg cctgccagcg ccgactcggg catgtgctgt gagagtgact 1141 cggagcccgg caccagtgtg tcggagcccc tcacccctga tgccgggagt cacaccttcg 1201 cctcagggcc agaggaaggg ctgagctacc tggcagcctc agaggctccg ctgccacctg 1261 ggggcccTGC GGAGGCCATC AAGGAGCATG AACGGTGGCT GGCCATGTGC ATCCAGGCCC 1321 TGCAGCGGGA AGTGGCAGAG GAGGACCTGG TGCAGGTGGA CAGAGCCGTG GACGCCCTCG 1381 ACCGCTGGGA GATGTTCACG GGCCAGCTCC CGGCCACCAG GCAGGACCCA CCCAGCAGCA 1441 GGGACAGCGT GGGGCTGCGC AAGGTGCTGG GGGACAAGTT CTCCTCCCTG AGGAGGAAGC 1501 TGAGTGCCCG AAAACTCGCC AGAGCAGAGT CGGCCCCCCG TCCCTGGGAT GGGGAAGAGA 1561 GTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1621 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1681 GGCTTTATAT ATCTTGTGGA AAGGACGAGC TCCAGGTTCC CAACCGATCT TCACGCGTTA 1741 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt