Construct: ORF TRCN0000476211
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015332.1_s317c1
- Derived from:
- ccsbBroadEn_08537
- DNA Barcode:
- ACGTAGAAAAATGCAGGTGATTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPRC5D (55507)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476211
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55507 | GPRC5D | G protein-coupled receptor ... | NM_018654.1 | 99.9% | 100% | 714C>T |
| 2 | human | 55507 | GPRC5D | G protein-coupled receptor ... | XM_005253421.3 | 99.9% | 100% | 714C>T |
| 3 | human | 55507 | GPRC5D | G protein-coupled receptor ... | XM_017019582.2 | 99.9% | 100% | 714C>T |
| 4 | human | 55507 | GPRC5D | G protein-coupled receptor ... | XM_017019583.2 | 86.7% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1101
- ORF length:
- 1035
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta caaggactgc atcgagtcca ctggagacta ttttcttctc tgtgacgccg 121 aggggccatg gggcatcatt ctggagtccc tggccatact tggcatcgtg gtcacaattc 181 tgctactctt agcatttctc ttcctcatgc gaaagatcca agactgcagc cagtggaatg 241 tcctccccac ccagctcctc ttcctcctga gtgtcctggg gctcttcgga ctcgcttttg 301 ccttcatcat cgagctcaat caacaaactg cccccgtacg ctactttctc tttggggttc 361 tctttgctct ctgtttctca tgcctcttag ctcatgcctc caatctagtg aagctggttc 421 ggggttgtgt ctccttctcc tggacgacaa ttctgtgcat tgctattggt tgcagtctgt 481 tgcaaatcat tattgccact gagtatgtga ctctcatcat gaccagaggt atgatgtttg 541 tgaatatgac accctgccag ctcaatgtgg actttgttgt actcctggtc tatgtcctct 601 tcctgatggc cctcacattc ttcgtctcca aagccacctt ctgtggcccg tgtgagaact 661 ggaagcagca tggaaggctc atctttatca ctgtgctctt ctccatcatc atctgggtgg 721 tgtggatctc catgctccTG AGAGGCAACC CGCAGTTCCA GCGACAGCCC CAGTGGGATG 781 ACCCGGTCGT CTGCATTGCT CTGGTCACCA ACGCATGGGT TTTCCTGCTG CTGTACATCG 841 TCCCTGAGCT CTGCATTCTC TACAGATCGT GTAGACAGGA GTGCCCTTTA CAAGGCAATG 901 CCTGCCCCGT CACAGCCTAC CAACACAGCT TCCAAGTGGA GAACCAGGAG CTCTCCAGAG 961 CCCGAGACAG TGATGGAGCT GAGGAGGATG TAGCATTAAC TTCATATGGT ACTCCCATTC 1021 AGCCGCAGAC TGTTGATCCC ACACAAGAGT GTTTCATCCC ACAGGCTAAA CTAAGCCCCC 1081 AGCAAGATGC AGGAGGAGTA TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1141 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1201 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACGT AGAAAAATGC 1261 AGGTGATTGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt