Construct: ORF TRCN0000476215
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010250.1_s317c1
- Derived from:
- ccsbBroadEn_03628
- DNA Barcode:
- ATAAGATACATTCAACATGCGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCYL2 (55681)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476215
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001317784.1 | 100% | 100% | |
| 2 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_017988.6 | 100% | 100% | |
| 3 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001330253.1 | 99.5% | 99.5% | 1272_1283del |
| 4 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001330254.1 | 99.5% | 99.5% | 1272_1283del |
| 5 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001330256.1 | 81% | 81% | 0_1ins519;753_764del |
| 6 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | NM_198021.2 | 88.7% | 93% | (many diffs) |
| 7 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | NM_001310704.1 | 88.7% | 92.9% | (many diffs) |
| 8 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | XM_011243394.2 | 88.4% | 92.6% | (many diffs) |
| 9 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | XM_006513458.3 | 88.3% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2853
- ORF length:
- 2787
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtccatgctt aataaattga agagtactgt tacaaaagta acagctgatg 121 tcactagtgc tgtaatggga aatcctgtca ctagagaatt tgatgttggt cgacacattg 181 ccagtggtgg caatgggcta gcttggaaga tttttaatgg cacaaaaaag tcaacaaagc 241 aggaagtggc agtttttgtc tttgataaaa aactgattga caagtatcaa aaatttgaaa 301 aggatcaaat cattgattct ctaaaacgag gagtccaaca gttaactcgg cttcgacacc 361 ctcgacttct tactgtccag catcctttag aagaatccag ggattgcttg gcattttgta 421 cagaaccagt ttttgccagt ttagccaatg ttcttggtaa ctgggaaaat ctaccttccc 481 ctatatctcc agacattaag gattataaac tttatgatgt agaaaccaaa tatggtttgc 541 ttcaggtttc tgaaggattg tcattcttgc atagcagtgt gaaaatggtg catggaaata 601 tcactcctga aaatataatt ttgaataaaa gtggagcctg gaaaataatg ggttttgatt 661 tttgtgtatc atcaaccaat ccttctgaac aagagcctaa atttccttgt aaagaatggg 721 acccaaattt accttcattg tgtcttccaa atcctgaata tttggctcct gaatacatac 781 tttctgtgag ctgtgaaaca gccagtgata tgtattcttt aggaactgtt atgtatgctg 841 tatttaataa agggaaacct atatttgaag tcaacaagca agatatttac aagagtttca 901 gtaggcagtt ggatcagttg agtcgtttag gatctagttc acttacaaat atacctgagg 961 aagttcgtga acatgtaaag ctactgttaa atgtaactcc gactgtaaga ccagatgcag 1021 atcaaatgac aaagattccc ttctttgatg atgttggtgc agtaacactg caatattttg 1081 ataccttatt ccaaagagat aatcttcaga aatcacagtt tttcaaagga ctgccaaagg 1141 ttctaccaaa actgcccaag cgtgtcattg tgcagagaat tttgccttgt ttgacttcag 1201 aatttgtaaa ccctgacatg gtaccttttg ttttgcccaa tgttctactt attgctgagg 1261 aatgcaccaa agaagaatat gtcaaattaa ttcttcctga acttggccct gtgtttaagc 1321 agcaggagcc aatccagatt ttgttaattt tcctacaaaa aatggatttg ctactaacca 1381 aaacccctcc tgatgagata aagaacagtg ttctacccat ggtttacaga gcactagaag 1441 ctccttccat tcagatccag gagctctgtc taaacatcat tccaaccttt gcaaatctta 1501 tagactaccc atccatgaaa aacgctttga taccaagaat taaaaatgct tgtctacaaa 1561 catcttccct tgcggttcgt gtaaattcat tagtgtgctt aggaaagatt ttggaatact 1621 tggataagtg gtttgtactt gatgatatcc tacccttctt acaacaaatt ccatccaagg 1681 aacctgcggt cctcatggga attttaggta tttacaaatg tacttttact cataagaagt 1741 tgggaatcac caaagagcag ctggccggaa aagtgttgcc tcatcttatt cccctgagta 1801 ttgaaaacaa tcttaatctt aatcagttca attctttcat ttccgtcata aaagaaatgc 1861 ttaatagatt ggagtctgaa cataagacta aactggagca acttcatata atgcaagaac 1921 agcagaaatc tttggatata ggaaatcaaa tgaatgtttc tgaggagatg aaagttacaa 1981 atattgggaa tcagcaaatt gacaaagttt ttaacaacat tggagcagac cttctgactg 2041 gcagtgagtc cgaaaataaa gaggacgggt tacagaataa acataaaaga gcatcactta 2101 cacttgaaga aaaacaaaaa ttagcaaaag aacaagagca ggcacagaag ctgaaaagcc 2161 agcagcctct taaaccccaa gtgcacacac ctgttgctac tgttaaacag actaaggact 2221 tgacagacac actgatggat aatatgtcat ccttgaccag cctttctgtt agtaccccta 2281 aatcttctgc ttcaagtact ttcacttctg ttccttccat gggcattggt atgatgtttt 2341 ctacaccaac tgataataca aagagaaatt tgacaaatgg cctaaatgcc aatatgggct 2401 ttcagacttc aggattcaac atgcccgtta atacaaacca gaacttctac agtagtccaa 2461 gcacagttgg agtgaccaag atgactctgg gaacacctcc cactttgcca aacttcaatg 2521 ctttgagtgt tccTCCTGCT GGTGCAAAGC AGACCCAACA AAGACCCACA GATATGTCTG 2581 CCCTTAATAA TCTCTTTGGC CCTCAGAAAC CCAAAGTTAG CATGAACCAG TTATCACAAC 2641 AGAAACCAAA TCAGTGGCTT AATCAGTTTG TACCTCCTCA AGGTTCTCCA ACTATGGGCA 2701 GTTCAGTAAT GGGGACACAG ATGAACGTGA TAGGACAATC TGCTTTTGGT ATGCAGGGTA 2761 ATCCTTTCTT TAACCCACAG AACTTTGCAC AGCCACCAAC TACTATGACC AATAGCAGTT 2821 CAGCTAGCAA TGATTTAAAA GATCTTTTTG GGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 2881 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2941 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 3001 AAGATACATT CAACATGCGT TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 3061 ttgtgaaaga tt