Construct: ORF TRCN0000476218
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015340.1_s317c1
- Derived from:
- ccsbBroadEn_12493
- DNA Barcode:
- TTTTGCCTTGAAACTGAACATGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRTC3 (64784)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476218
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64784 | CRTC3 | CREB regulated transcriptio... | XM_024450018.1 | 74.7% | 74.7% | 1_324del |
| 2 | human | 64784 | CRTC3 | CREB regulated transcriptio... | XM_024450019.1 | 74.7% | 74.7% | 1_324del |
| 3 | human | 64784 | CRTC3 | CREB regulated transcriptio... | XM_011521906.2 | 54.6% | 54.6% | 1_798del |
| 4 | human | 64784 | CRTC3 | CREB regulated transcriptio... | XM_005254968.2 | 51.7% | 51.7% | 1_894del |
| 5 | human | 64784 | CRTC3 | CREB regulated transcriptio... | NM_022769.5 | 51.6% | 51.6% | 1_897del |
| 6 | human | 64784 | CRTC3 | CREB regulated transcriptio... | NM_001042574.3 | 51.5% | 51.5% | 1_897del;1649_1650insAGA |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1029
- ORF length:
- 960
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtgtgggg aatagtgtga acaacatccc agctgctatg acccacctgg 121 gtataagaag ctcctctggt ctccagagtt ctcggagtaa cccctccatc caagccacgc 181 tcaataagac tgtgctttcc tcttccttaa ataaccaccc acagacatct gttcccaacg 241 catctgctct tcacccttcg ctccgtctgt tttcccttag caacccatct ctttccacca 301 caaacctgag cggcccgtct cggcgtcggc agcctcccgt cagccctctc acgctttctc 361 ctggccctga agcacatcaa ggtttcagca gacagctgtc ttcaaccagc ccactggccc 421 catatcctac ctcccagatg gtgtcctcag accgaagcca actttccttt ctgcccacag 481 aagctcaagc ccaggtgtcg ccgccacccc cttaccctgc accccaggag ctcacccagc 541 ccctcctgca gcagccccgc gcccctgagg cccctgccca gcagccccag gcagcctcct 601 cactgccaca gtcagacttt cagcttctcc cggcccaggg ctcatctttg accaacttct 661 tcccagatgt gggttttgac cagcagtcca tgaggccagg ccctgccttt cctcaacagg 721 tgcctcTGGT GCAACAAGGT TCCCGAGAAC TGCAGGACTC TTTTCATTTG AGACCAAGCC 781 CGTATTCCAA CTGCGGGAGT CTCCCGAACA CCATCCTGCC AGAAGACTCC AGCACCAGCC 841 TGTTCAAAGA CCTCAACAGT GCGCTGGCAG GCCTGCCTGA GGTCAGCCTG AACGTGGACA 901 CTCCATTTCC ACTGGAAGAG GAGCTGCAGA TTGAACCCCT GAGCCTGGAC GGACTCAACA 961 TGTTAAGTGA CTCCAGCATG GGCCTGCTGG ACCCCTCTGT TGAAGAGACG TTTCGAGCTG 1021 ACAGACTGTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTTTGC CTTGAAACTG AACATGGAAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt