Construct: ORF TRCN0000476254
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017770.1_s317c1
- Derived from:
- ccsbBroadEn_05641
- DNA Barcode:
- AAGGTCCTGATTCGCTAAACGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C2orf66 (401027)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476254
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 401027 | C2orf66 | chromosome 2 open reading f... | NM_213608.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 420
- ORF length:
- 351
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatcattgac agctcccgca TCCCCTCTTT CACTCAGCTT CACTCCACCA 121 TGACCAGAGC ACCTCTCCTG CTACTATGTG TTGCCCTGGT GCTGCTTGGG CATGTGAATG 181 GAGCCACAGT AAGAAATGAG GACAAATGGA AGCCACTCAA CAACCCCAGA AACAGAGATC 241 TGTTTTTCAG AAGGCTTCAG GCATATTTTA AGGGCAGAGG TCTTGATCTT GGAACATTTC 301 CAAATCCTTT CCCCACGAAT GAAAATCCTA GACCTCTCTC TTTCCAGTCA GAACTTACTG 361 CTTCTGCATC TGCAGATTAT GAAGAGCAGA AAAACTCCTT TCACAATTAT CTCAAAGGCT 421 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 481 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 541 TTTATATATC TTGTGGAAAG GACGAAAGGT CCTGATTCGC TAAACGTGCA CGCGTTAAGT 601 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt