Construct: ORF TRCN0000476278
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003339.1_s317c1
- Derived from:
- ccsbBroadEn_05851
- DNA Barcode:
- GCATTAGTCACCGACGTAGTGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STS (412)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476278
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 412 | STS | steroid sulfatase | NM_000351.7 | 99% | 99.1% | 0_1insATGCCTTTAAGGAAG;1272G>A |
| 2 | human | 412 | STS | steroid sulfatase | NM_001320752.2 | 99% | 99.1% | 0_1insATGCCTTTAAGGAAG;1272G>A |
| 3 | human | 412 | STS | steroid sulfatase | NM_001320753.1 | 99% | 99.1% | 0_1insATGCCTTTAAGGAAG;1272G>A |
| 4 | human | 412 | STS | steroid sulfatase | NM_001320754.1 | 99% | 99.1% | 0_1insATGCCTTTAAGGAAG;1272G>A |
| 5 | human | 412 | STS | steroid sulfatase | NM_001320750.2 | 98.5% | 98.1% | (many diffs) |
| 6 | human | 412 | STS | steroid sulfatase | NM_001320751.1 | 98.5% | 98.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1818
- ORF length:
- 1749
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctttaagg aagatgaaga tccctttcct cctactgttc tttctgtggg 121 aagccgagag ccacgcagca tcaaggccga acatcatcct ggtgatggct gacgacctcg 181 gcattggaga tcctgggtgc tatgggaaca aaactatcag gactcccaat atcgaccggt 241 tggccagtgg gggagtgaaa ctcactcagc acctggcagc atcaccgctg tgcacaccaa 301 gcagggcagc cttcatgact ggccggtacc ctgtccgatc aggaatggca tcttggtccc 361 gcactggagt tttcctcttc acagcctctt cgggaggact tcccaccgat gagattacct 421 ttgctaagct tctgaaggat caaggttatt caacagcact gatagggaaa tggcaccttg 481 ggatgagctg tcacagcaag actgacttct gtcaccaccc tttacatcac ggcttcaatt 541 atttctatgg gatctctttg accaatctga gagactgcaa gcccggagag ggcagtgtct 601 tcaccacggg cttcaagagg ctggtcttcc tccccctgca gatcgtcggg gtcaccctcc 661 ttacccttgc tgcactcaat tgtctggggc tactccacgt gcctctaggc gtttttttca 721 gccttctctt cctagcagcc ctaatcctga cccttttctt gggcttcctt cattacttcc 781 ggcccctgaa ctgcttcatg atgaggaact acgagatcat tcagcagccc atgtcctatg 841 acaatctcac ccagaggcta acggtggagg cggcccagtt catacagcgg aacactgaga 901 ctccgttcct gcttgtcttg tcctacctcc acgtgcacac agccctgttc tccagcaaag 961 actttgctgg caaaagtcaa cacggagtct acggggatgc tgttgaggaa atggactgga 1021 gtgtggggca gatcttgaac cttctggatg agctgagatt ggctaatgat accctcatct 1081 acttcacatc ggaccaggga gcacatgtag aagaagtgtc ttccaaagga gaaattcatg 1141 gcggaagtaa tgggatctat aaaggaggaa aagcaaacaa ctgggaagga ggtatccggg 1201 ttccaggcat ccttcgttgg cccagggtga tacaggctgg ccagaagatt gatgagccca 1261 ctagcaacat ggacatattt cctacagtag ccaagctggc tggagctccc ttgcctgagg 1321 acaggatcat tgatggacgt gatctgatgc ccctacttga aggaaaaagc caacgctccg 1381 atcatgagtt tctcttccat tactgcaacg cctacttaaa tgctgtgcgc tggcaccctc 1441 agaacagcac atccatctgg aaggcctttT TCTTCACCCC CAACTTCAAC CCCGTGGGTT 1501 CCAACGGATG CTTTGCCACA CACGTGTGCT TCTGTTTCGG GAGTTATGTC ACCCATCACG 1561 ACCCACCTTT ACTCTTTGAT ATTTCCAAAG ATCCCAGAGA GAGAAACCCA CTAACTCCAG 1621 CATCCGAGCC CCGGTTTTAT GAAATCCTCA AAGTCATGCA GGAAGCTGCG GACAGACACA 1681 CCCAGACCCT GCCAGAGGTG CCCGATCAGT TTTCATGGAA CAACTTTCTT TGGAAGCCCT 1741 GGCTTCAGCT GTGCTGTCCT TCCACCGGCC TGTCTTGCCA GTGTGATAGA GAAAAACAGG 1801 ATAAGAGACT GAGCCGCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1861 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1921 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCATTAG TCACCGACGT 1981 AGTGGCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt