Construct: ORF TRCN0000476281
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000980.1_s317c1
- Derived from:
- ccsbBroadEn_06746
- DNA Barcode:
- AGCTGATAATTAAGCTTACCCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PODXL (5420)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476281
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5420 | PODXL | podocalyxin like | NM_005397.4 | 99.9% | 100% | 231G>A |
| 2 | human | 5420 | PODXL | podocalyxin like | NM_001018111.3 | 94.2% | 94% | 231G>A;707_802del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1647
- ORF length:
- 1578
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgctgcgcg ctggcgctct cggcgctgct gctactgttg tcaacgccgc 121 cgctgctgcc gtcgtcgccg tcgccgtcgc cgtcgccctc ccagaatgca acccagacta 181 ctacggactc atctaacaaa acagcaccga ctccagcatc cagtgtcacc atcatggcta 241 cagatacagc ccagcagagc acagtcccca cttccaaggc caacgaaatc ttggcctcag 301 tcaaggcgac cacccttggt gtatccagtg actcaccggg gactacaacc ctggctcagc 361 aagtctcagg cccagtcaac actaccgtgg ctagaggagg cggctcaggc aaccctacta 421 ccaccatcga gagccccaag agcacaaaaa gtgcagacac cactacagtt gcaacctcca 481 cagccacagc taaacctaac accacaagca gccagaatgg agcagaagat acaacaaact 541 ctggggggaa aagcagccac agtgtgacca cagacctcac atccactaag gcagaacatc 601 tgacgacccc tcaccctaca agtccactta gcccccgaca acccacttcg acgcatcctg 661 tggccacccc aacaagctcg ggacatgacc atcttatgaa aatttcaagc agttcaagca 721 ctgtggctat ccctggctac accttcacaa gcccggggat gaccaccacc ctaccgtcat 781 cggttatctc gcaaagaact caacagacct ccagtcagat gccagccagc tctacggccc 841 cttcctccca ggagacagtg cagcccacga gcccggcaac ggcattgaga acacctaccc 901 tgccagagac catgagctcc agccccacag cagcatcaac tacccaccga taccccaaaa 961 caccttctcc cactgtggct catgagagta actgggcaaa gtgtgaggat cttgagacac 1021 agacacagag tgagaagcag ctcgtcctga acctcacagg aaacaccctc tgtgcagggg 1081 gcgcttcgga tgagaaattg atctcactga tatgccgagc agtcaaagcc accttcaacc 1141 cggcccaaga taagtgcggc atacggctgg catctgttcc aggaagtcag accgtggtcg 1201 tcaaagaaat cactattcac actaagctcc ctgccaagga tgtgtacgag cggctgaagg 1261 acaaatggga tgaactaaag gaggcagggg tCAGTGACAT GAAGCTAGGG GACCAGGGGC 1321 CACCGGAGGA GGCCGAGGAC CGCTTCAGCA TGCCCCTCAT CATCACCATC GTCTGCATGG 1381 CATCATTCCT GCTCCTCGTG GCGGCCCTCT ATGGCTGCTG CCACCAGCGC CTCTCCCAGA 1441 GGAAGGACCA GCAGCGGCTA ACAGAGGAGC TGCAGACAGT GGAGAATGGT TACCATGACA 1501 ACCCAACACT GGAAGTGATG GAGACCTCTT CTGAGATGCA GGAGAAGAAG GTGGTCAGCC 1561 TCAACGGGGA GCTGGGGGAC AGCTGGATCG TCCCTCTGGA CAACCTGACC AAGGACGACC 1621 TGGATGAGGA GGAAGACACA CACCTCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1681 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1741 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGCTGATA 1801 ATTAAGCTTA CCCTCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1861 aagatt