Construct: ORF TRCN0000476295
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011596.1_s317c1
- Derived from:
- ccsbBroadEn_08783
- DNA Barcode:
- ACGGACGCTTTTCTTTGTCTCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IL22RA1 (58985)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476295
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58985 | IL22RA1 | interleukin 22 receptor sub... | NM_021258.4 | 99.8% | 100% | 435A>C;936C>T |
| 2 | human | 58985 | IL22RA1 | interleukin 22 receptor sub... | XM_011541882.1 | 76.1% | 76.3% | 0_1ins408;27A>C;528C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1788
- ORF length:
- 1722
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gacgctgctg accatcttga ctgtgggatc cctggctgct cacgcccctg 121 aggacccctc ggatctgctc cagcacgtga aattccagtc cagcaacttt gaaaacatcc 181 tgacgtggga cagcgggccg gagggcaccc cagacacggt ctacagcatc gagtataaga 241 cgtacggaga gagggactgg gtggcaaaga agggctgtca gcggatcacc cggaagtcct 301 gcaacctgac ggtggagacg ggcaacctca cggagctcta ctatgccagg gtcaccgctg 361 tcagtgcggg aggccggtca gccaccaaga tgactgacag gttcagctct ctgcagcaca 421 ctaccctcaa gccacctgat gtgacctgta tctccaaagt gagatcgatt cagatgattg 481 ttcatcctac ccccacgccc atccgtgcag gcgatggcca ccggctaacc ctggaagaca 541 tcttccatga cctgttctac cacttagagc tccaggtcaa ccgcacctac caaatgcacc 601 ttggagggaa gcagagagaa tatgagttct tcggcctgac ccctgacaca gagttccttg 661 gcaccatcat gatttgcgtt cccacctggg ccaaggagag tgccccctac atgtgccgag 721 tgaagacact gccagaccgg acatggacct actccttctc cggagccttc ctgttctcca 781 tgggcttcct cgtcgcagta ctctgctacc tgagctacag atatgtcacc aagccgcctg 841 cacctcccaa ctccctgaac gtccagcgag tcctgacttt ccagccgctg cgcttcatcc 901 aggagcacgt cctgatccct gtctttgacc tcagcggccc cagcagtctg gcccagcctg 961 tccagtactc ccagatcagg gtgtctggac ccagggagcc tgcaggagct ccacagcggc 1021 atagcctgtc cgagatcacc tacttagggc agccagacat ctccatcctc cagccctcca 1081 acgtgccacc tccccagatc ctctccccac tgtcctatgc cccaaacgct gcccctgagg 1141 tcgggccccc atcctatgca cctcaggtga cccccgaagc tcaattccca ttctacgccc 1201 cacaggccat ctctaaggtc cagccttcct cctatgcccc tcaagccact ccggacagct 1261 ggcctccctc ctatggggta tgcatggaag gttctggcaa agactccccc actgggacac 1321 tttctagtcc taaacacctt aggcctaaag gtcagcttca gaaagagcca ccagctggaa 1381 gctgcatgtt aggtggcctt tctctgcagg aggtgacctc cttggctatg gaggaatccc 1441 aagaagcaaa atcattgcac cagcccctgg ggatttgcac agacagaaca tctgacccaa 1501 atgtgctaca cagtggggag gaagggacac cacagtaccT AAAGGGCCAG CTCCCCCTCC 1561 TCTCCTCAGT CCAGATCGAG GGCCACCCCA TGTCCCTCCC TTTGCAACCT CCTTCCCGTC 1621 CATGTTCCCC CTCGGACCAA GGTCCAAGTC CCTGGGGCCT GCTGGAGTCC CTTGTGTGTC 1681 CCAAGGATGA AGCCAAGAGC CCAGCCCCTG AGACCTCAGA CCTGGAGCAG CCCACAGAAC 1741 TGGATTCTCT TTTCAGAGGC CTGGCCCTGA CTGTGCAGTG GGAGTCCTGC CCAACTTTCT 1801 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1861 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1921 GTGGAAAGGA CGAACGGACG CTTTTCTTTG TCTCGTCACG CGTTAAGTCg acaatcaacc 1981 tctggattac aaaatttgtg aaagatt