Construct: ORF TRCN0000476298
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015709.1_s317c1
- Derived from:
- ccsbBroadEn_08915
- DNA Barcode:
- AGGTATTTTGGCCACCCATCGCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASPSCR1 (79058)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476298
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | NM_024083.4 | 99.8% | 99.8% | 755T>A;1461T>C |
| 2 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | XM_017025042.1 | 95.5% | 95.3% | 755T>A;1301_1375del;1536T>C |
| 3 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | NR_045351.2 | 90.7% | (many diffs) | |
| 4 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | XM_011523602.3 | 88.4% | 86.4% | (many diffs) |
| 5 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | XM_011523601.3 | 86.9% | 86.2% | (many diffs) |
| 6 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | XM_017025044.1 | 85.9% | 85.8% | 0_1ins231;524T>A;1230T>C |
| 7 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | NM_001251888.2 | 85.3% | 85.3% | 755T>A;1353_1634del;1743T>C |
| 8 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | XM_017025037.2 | 85% | 82.9% | (many diffs) |
| 9 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | XM_017025036.2 | 83.6% | 82.8% | (many diffs) |
| 10 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | NM_001330528.2 | 82.2% | 82% | (many diffs) |
| 11 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | XR_001752618.2 | 81.7% | (many diffs) | |
| 12 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | XM_024450926.1 | 76% | 74.1% | (many diffs) |
| 13 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | XM_017025038.2 | 72% | 71.2% | (many diffs) |
| 14 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | XM_017025039.2 | 72% | 71.2% | (many diffs) |
| 15 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | XM_017025040.2 | 72% | 71.2% | (many diffs) |
| 16 | human | 79058 | ASPSCR1 | ASPSCR1 tether for SLC2A4, ... | XM_017025041.2 | 72% | 71.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1725
- ORF length:
- 1659
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggccccggca ggcggcggag gctccgcggt gtcggtgctg gccccgaacg 121 gccggcgcca cacggtgaag gtgacgccga gcaccgtgct gcttcaggtt ctggaggaca 181 cgtgccggcg gcaggacttc aacccctgtg aatatgatct gaagtttcag aggagcgtgc 241 tcgacctttc tctccagtgg agatttgcca acctgcccaa caatgccaag ctggagatgg 301 tgcccgcttc ccggagccgt gaggggcctg agaacatggt tcgcatcgct ttgcagctgg 361 acgatggctc gaggttgcag gactctttct gttcaggcca gaccctctgg gagcttctca 421 gccattttcc acagatcagg gagtgcctgc agcaccccgg cggggccacc ccagtctgcg 481 tgtacacgag ggatgaggtg acgggtgaag ctgccctgcg gggcacgacg ctgcagtcgc 541 tgggcctgac cgggggcagc gccaccatca ggtttgtcat gaagtgctac gaccccgtgg 601 gcaagacccc aggaagcctg ggctcgtcag cgtcggctgg ccaggcagcc gccagcgctc 661 cacttccctt ggaatctggg gagctcagcc gcggcgactt gagccgtccg gaggacgcgg 721 acacctcagg gccctgctgc gagcacactc aggagaagca gagcacaagg gcacccgcag 781 ctgccccctt tgttcctttc tcgggtgggg gacagagaca ggggggccct cctgggccca 841 cgaggcctct gacatcatct tcagctaagt tgccgaagtc cctctccagc cctggaggcc 901 cctccaagcc aaagaagtcc aagtcgggcc aggatcccca gcaggagcag gagcaggagc 961 gggagcggga tccccagcag gagcaggagc gggagcggcc cgtggaccgg gagcccgtgg 1021 accgggagcc ggtggtgtgc caccccgacc tggaggagcg gctgcaggcc tggccagcgg 1081 agctgcctga tgagttcttt gagctgacgg tggacgacgt gagaagacgc ttggcccagc 1141 tcaagagtga gcggaagcgc ctggaagaag cccccttggt gaccaaggcc ttcagggagg 1201 cgcagataaa ggagaagctg gagcgctacc caaaggtggc tctgagggtc ctgttccccg 1261 accgctacgt cctacagggc ttcttccgcc ccagcgagac agtgggggac ttgcgagact 1321 tcgtgaggag ccacctgggg aaccccgagc tgtcatttta cctgttcatc acccctccaa 1381 aaacagtcct ggacgaccac acgcagaccc tctttCAGGC GAACCTCTTC CCGGCCGCTC 1441 TGGTGCACTT GGGAGCCGAG GAGCCGGCAG GTGTCTACCT GGAGCCTGGC CTGCTGGAGC 1501 ATGCCATCTC CCCATCTGCG GCCGACGTGC TGGTGGCCAG GTACATGTCC AGGGCCGCCG 1561 GGTCCCCTTC CCCATTGCCA GCCCCTGACC CTGCACCTAA GTCTGAGCCA GCTGCTGAGG 1621 AGGGGGCGCT GGTCCCCCCT GAGCCCATCC CAGGGACGGC CCAGCCCGTG AAGAGGAGCC 1681 TGGGCAAGGT GCCCAAGTGG CTGAAGCTGC CGGCCAGCAA GAGGTGCCCA ACTTTCTTGT 1741 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1801 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1861 GAAAGGACGA AGGTATTTTG GCCACCCATC GCGAACGCGT TAAGTCgaca atcaacctct 1921 ggattacaaa atttgtgaaa gatt