Transcript: Human XM_017025039.2

PREDICTED: Homo sapiens ASPSCR1 tether for SLC2A4, UBX domain containing (ASPSCR1), transcript variant X6, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ASPSCR1 (79058)
Length:
2129
CDS:
257..2008

Additional Resources:

NCBI RefSeq record:
XM_017025039.2
NBCI Gene record:
ASPSCR1 (79058)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017025039.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000320840 TCGAGGTTGCAGGACTCTTTC pLKO_005 329 CDS 100% 10.800 15.120 N ASPSCR1 n/a
2 TRCN0000320841 CTTCAGGGAGGCGCAGATAAA pLKO_005 1150 CDS 100% 13.200 9.240 N ASPSCR1 n/a
3 TRCN0000368934 CTTTCTCTCCAGTGGAGATTT pLKO_005 206 5UTR 100% 13.200 9.240 N ASPSCR1 n/a
4 TRCN0000022414 CCAGCAGGAATAAAGACTTGT pLKO.1 1835 CDS 100% 4.950 3.465 N ASPSCR1 n/a
5 TRCN0000320911 CCAGCAGGAATAAAGACTTGT pLKO_005 1835 CDS 100% 4.950 3.465 N ASPSCR1 n/a
6 TRCN0000022418 GCCTGATGAGTTCTTTGAGCT pLKO.1 1045 CDS 100% 2.640 1.848 N ASPSCR1 n/a
7 TRCN0000022417 CCTGCACCTAAGTCTGAGCCA pLKO.1 1625 CDS 100% 0.220 0.154 N ASPSCR1 n/a
8 TRCN0000022416 GAGCTGCCTGATGAGTTCTTT pLKO.1 1040 CDS 100% 5.625 3.375 N ASPSCR1 n/a
9 TRCN0000181657 GCCACCATCAGGTTTGTCATA pLKO.1 521 CDS 100% 4.950 6.930 N Aspscr1 n/a
10 TRCN0000277301 GCCACCATCAGGTTTGTCATA pLKO_005 521 CDS 100% 4.950 6.930 N Aspscr1 n/a
11 TRCN0000022415 CCTGTGAATATGATCTGAAGT pLKO.1 38 5UTR 100% 4.950 3.465 N ASPSCR1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017025039.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08915 pDONR223 100% 72% 71.2% None (many diffs) n/a
2 ccsbBroad304_08915 pLX_304 0% 72% 71.2% V5 (many diffs) n/a
3 TRCN0000476298 AGGTATTTTGGCCACCCATCGCGA pLX_317 19% 72% 71.2% V5 (many diffs) n/a
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