Construct: ORF TRCN0000476329
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013025.1_s317c1
- Derived from:
- ccsbBroadEn_07899
- DNA Barcode:
- GAACCGATCGCACTTTGGTTACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HYAL4 (23553)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476329
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23553 | HYAL4 | hyaluronidase 4 | NM_012269.3 | 99.8% | 99.7% | 867C>T;1036G>T |
| 2 | human | 23553 | HYAL4 | hyaluronidase 4 | XM_011515990.2 | 99.8% | 99.7% | 867C>T;1036G>T |
| 3 | human | 23553 | HYAL4 | hyaluronidase 4 | XM_017011911.1 | 99.8% | 99.7% | 867C>T;1036G>T |
| 4 | human | 23553 | HYAL4 | hyaluronidase 4 | XM_024446703.1 | 93.6% | 93.7% | 867C>T;951_952ins90 |
| 5 | human | 23553 | HYAL4 | hyaluronidase 4 | XR_002956416.1 | 71.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1512
- ORF length:
- 1443
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaagtatta tctgaaggac agttaaagct ttgtgttgtt caaccagtac 121 atctcacttc atggctcctt atatttttta ttctaaagtc tatctcttgt ctaaaacctg 181 ctcgacttcc aatttatcaa aggaaacctt ttatagctgc ttggaatgct ccaacagatc 241 agtgtttgat aaaatataat ttaagactaa atttgaaaat gtttcctgtg attggaagcc 301 cactggccaa ggccaggggg caaaatgtca ctatatttta tgtcaacaga ttgggatact 361 atccgtggta tacatcacaa ggggtcccca ttaatggagg tctcccacag aacataagtt 421 tacaagtaca tctggaaaaa gctgaccaag atattaatta ttacatccct gctgaagatt 481 tcagtggact tgctgttata gattgggaat attggcgacc acagtgggcc cggaactgga 541 actcaaaaga tgtttacaga cagaagtcaa gaaagcttat ttccgatatg ggaaagaatg 601 tatcagctac cgatattgaa tatttagcca aagtgacctt tgaagaaagt gcaaaagctt 661 tcatgaagga aaccatcaaa ttgggaatta agagccgacc caaaggcctt tggggttatt 721 atttatatcc tgattgccac aattataacg tttatgcccc aaactactct gggtcatgcc 781 cagaagacga agtcttgagg aacaatgagc tctcttggct ctggaacagc agtgctgctt 841 tatatccttc tatcggtgtc tggaaatccc ttggagacag tgaaaacatt ttgcgcttct 901 ccaaatttcg ggtgcatgaa tccatgagga tctctaccat gacatctcat gattatgctc 961 tgcctgtatt tgtctacaca aggctagggt acagagatga acctttattt ttcctttcta 1021 agcaagatct agtcagcacc ataggagaaa gtgctgcctt gggagctgca ggcattgtta 1081 tttggggaga catgaattta acttcatcca aggccaactg tacaaaggtg aagcagtttg 1141 tgagttctga tttagggagc tacatagcca atgtgaccag agctgctgag gtatgcagcc 1201 TTCACCTCTG CAGGAACAAT GGCAGGTGCA TAAGGAAGAT GTGGAACGCG CCCAGTTACC 1261 TTCACTTGAA CCCTGCAAGT TACCACATAG AGGCCTCTGA GGACGGGGAG TTTACTGTGA 1321 AAGGAAAAGC ATCTGATACA GACCTGGCAG TGATGGCAGA TACATTTTCC TGTCATTGTT 1381 ATCAGGGATA TGAAGGAGCT GATTGCAGAG AAATAAAGAC GGCTGATGGC TGCTCTGGGG 1441 TTTCCCCTTC TCCTGGTTCA CTAATGACAC TTTGTCTACT GCTTTTAGCA AGTTATCGAA 1501 GCATTCAGTT GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1561 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1621 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGAA CCGATCGCAC TTTGGTTACC 1681 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t