Construct: ORF TRCN0000476330
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010455.1_s317c1
- Derived from:
- ccsbBroadEn_15128
- DNA Barcode:
- CCCCCTATGACATTCAAGTCAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNK15 (60598)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476330
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60598 | KCNK15 | potassium two pore domain c... | NM_022358.4 | 100% | 100% | |
| 2 | human | 60598 | KCNK15 | potassium two pore domain c... | XM_017028003.1 | 32.5% | 25.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1059
- ORF length:
- 990
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcggaggccg agcgtgcgcg cggccgggct ggtcctgtgc accctgtgtt 121 acctgctggt gggcgctgct gtcttcgacg cgctcgagtc cgaggcggaa agcggccgcc 181 agcgactgct ggtccagaag cggggcgctc tccggaggaa gttcggcttc tcggccgagg 241 actaccgcga gctggagcgc ctggcgctcc aggctgagcc ccaccgcgcc ggccgccagt 301 ggaagttccc cggctccttc tacttcgcca tcaccgtcat cactaccatc gagtacggcc 361 acgccgcgcc gggtacggac tccggcaagg tcttctgcat gttctacgcg ctcctgggca 421 tcccgctgac gctggtcact ttccagagcc tgggcgaacg gctgaacgcg gtggtgcggc 481 gcctcctgtt ggcggccaag tgctgcctgg gcctgcggtg gacgtgcgtg tccacggaga 541 acctggtggt ggccgggctg ctggcgtgtg ccgccaccct ggccctcggg gccgtcgcct 601 tctcgcactt cgagggctgg accttcttcc acgcctacta ctactgcttc atcaccctca 661 ccaccatcgg cttcggcgac ttcgtggcac tgcagagcgg cgaggcgctg cagaggaagc 721 tcccctacgt ggccttcagc ttcctctaca tcctccTGGG GCTCACGGTC ATTGGCGCCT 781 TCCTCAACCT GGTGGTCCTG CGCTTCCTCG TTGCCAGCGC CGACTGGCCC GAGCGCGCTG 841 CCCGCACCCC CAGCCCGCGC CCCCCGGGGG CGCCCGAGAG CCGTGGCCTC TGGCTGCCCC 901 GCCGCCCGGC CCGCTCCGTG GGCTCCGCCT CTGTCTTCTG CCACGTGCAC AAGCTGGAGA 961 GGTGCGCCCG CGACAACCTG GGCTTTTCGC CCCCCTCGAG CCCGGGGGTC GTGCGTGGCG 1021 GGCAGGCTCC CAGGCTTGGG GCCCGGTGGA AGTCCATCTT GCCAACTTTC TTGTACAAAG 1081 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1141 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1201 ACGACCCCCT ATGACATTCA AGTCAAAGAC GCGTTAAGTC gacaatcaac ctctggatta 1261 caaaatttgt gaaagatt