Construct: ORF TRCN0000476344

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007145.1_s317c1
Derived from:
ccsbBroadEn_04770
DNA Barcode:
TTCCGTGATGCGAGACGAATTCGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LEO1 (123169)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476344
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 123169 LEO1 LEO1 homolog, Paf1/RNA poly... NM_138792.4 100% 100%
2 human 123169 LEO1 LEO1 homolog, Paf1/RNA poly... NM_001323904.2 98.1% 95.6% 1894_1895insAGGA;1962_1963ins32
3 human 123169 LEO1 LEO1 homolog, Paf1/RNA poly... XM_017021911.1 95.9% 94% (many diffs)
4 human 123169 LEO1 LEO1 homolog, Paf1/RNA poly... NM_001323903.1 93.7% 92.7% 1895_1992del;2064_2065ins32
5 human 123169 LEO1 LEO1 homolog, Paf1/RNA poly... NM_001286430.1 90.9% 90.9% 1158_1159ins180
6 mouse 235497 Leo1 Leo1, Paf1/RNA polymerase I... NM_001039522.1 86.8% 93.5% (many diffs)
7 mouse 235497 Leo1 Leo1, Paf1/RNA polymerase I... XM_017313344.1 85.2% 89.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2064
ORF length:
1998
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggatatggag gatctcttcg ggagcgacgc cgacagcgaa gctgagcgta
121aagattctga ttctggatct gactcagatt ctgatcaaga gaatgctgcc tctggcagta
181atgcctctgg aagtgaaagt gatcaggatg aaagaggtga ttcaggacaa ccaagtaata
241aggaactgtt tggagatgac agtgaggacg agggagcttc acatcatagt ggtagtgata
301atcactctga aagatcagac aatagatcag aagcttctga gcgttctgac catgaggaca
361atgacccctc agatgtagat cagcacagtg gatcagaagc ccctaatgat gatgaagacg
421aaggtcatag atcggatgga gggagccatc attcagaagc agaaggttct gaaaaagcac
481attcagatga tgaaaaatgg ggcagagaag ataaaagtga ccagtcagat gatgaaaaga
541tacaaaattc tgatgatgag gagagggcac aaggatctga tgaagataag ctgcagaatt
601ctgacgatga tgagaaaatg cagaacacag atgatgagga gaggcctcag ctttccgatg
661atgagagaca acagctatct gaggaggaaa aggctaattc tgatgatgaa cggccggtag
721cttctgataa tgatgatgag aaacagaatt ctgatgatga agaacaacca cagctgtctg
781atgaagagaa aatgcaaaat tctgatgatg aaaggccaca ggcctcagat gaagaacaca
841ggcattcaga tgatgaagag gaacaggatc ataaatcaga atctgcaaga ggcagtgata
901gtgaagatga agttttacga atgaaacgca agaatgcgat tgcatctgat tcagaagcgg
961atagtgacac tgaggtgcca aaagataata gtggaaccat ggatttattt ggaggtgcag
1021atgatatctc ttcagggagt gatggagaag acaaaccacc tactccagga cagcctgttg
1081atgaaaatgg attgcctcag gatcaacagg aagaggagcc aattcctgag accagaatag
1141aagtagaaat acccaaagta aacactgatt taggaaacga cttatatttt gttaaactgc
1201ccaactttct cagtgtagag cccagacctt ttgatcctca gtattatgaa gatgaatttg
1261aagatgaaga aatgctggat gaagaaggta gaaccaggtt aaaattaaag gtagaaaata
1321ctataagatg gaggatacgc cgagatgaag aaggaaatga aattaaagaa agcaatgctc
1381ggatagtcaa gtggtcagat ggaagcatgt ccctgcattt aggcaatgaa gtgtttgatg
1441tgtacaaagc cccactgcag ggcgaccaca atcatctttt tataagacaa ggtactggtc
1501tacagggaca agcagtcttt aaaacgaaac tcaccttcag acctcactct acggacagtg
1561ccacacatag aaagatgact ctgtcacttg cagataggtg ttcaaagaca cagaagatta
1621gaatcttgcc aatggctggt cgtgatcctg aatgccaacg cacagaaatg attaagaaag
1681aagaagaacg tttGAGGGCT TCCATACGTA GGGAATCTCA GCAGCGCCGA ATGAGAGAGA
1741AACAGCACCA GCGGGGGCTG AGCGCCAGTT ACCTGGAACC TGATCGATAC GATGAGGAGG
1801AGGAAGGCGA GGAGTCCATC AGCTTGGCTG CCATTAAAAA CCGATATAAA GGGGGCATTC
1861GAGAGGAACG AGCCAGAATC TATTCATCAG ACAGTGATGA GGGATCAGAA GAAGATAAAG
1921CTCAAAGATT ACTCAAAGCA AAGAAACTTA CCAGTGATGA GGAAGGTGAA CCTTCCGGAA
1981AGAGAAAAGC AGAAGATGAT GATAAAGCAA ATAAAAAGCA TAAGAAGTAT GTGATCAGCG
2041ATGAAGAGGA AGAAGATGAT GATTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
2101AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
2161TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT TCCGTGATGC
2221GAGACGAATT CGCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
2281att

Download FASTA (ORF) (Full)