Construct: ORF TRCN0000476352
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002770.1_s317c1
- Derived from:
- ccsbBroadEn_07098
- DNA Barcode:
- ACAACCACCACATGATTTTCTTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRAF3 (7187)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476352
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7187 | TRAF3 | TNF receptor associated fac... | NM_003300.4 | 99.9% | 99.8% | 14A>G |
| 2 | human | 7187 | TRAF3 | TNF receptor associated fac... | NM_145725.3 | 99.9% | 99.8% | 14A>G |
| 3 | human | 7187 | TRAF3 | TNF receptor associated fac... | XM_017021617.1 | 99.9% | 99.8% | 14A>G |
| 4 | human | 7187 | TRAF3 | TNF receptor associated fac... | XM_017021618.1 | 99.9% | 99.8% | 14A>G |
| 5 | human | 7187 | TRAF3 | TNF receptor associated fac... | NM_145726.2 | 95.5% | 95.4% | 14A>G;649_650ins75 |
| 6 | human | 7187 | TRAF3 | TNF receptor associated fac... | XM_017021619.1 | 95.5% | 95.4% | 14A>G;649_650ins75 |
| 7 | human | 7187 | TRAF3 | TNF receptor associated fac... | XM_011537116.3 | 90.7% | 90.6% | 14A>G;567_568ins156 |
| 8 | human | 7187 | TRAF3 | TNF receptor associated fac... | XM_011537117.3 | 90% | 89.9% | 14A>G;649_650ins168 |
| 9 | human | 7187 | TRAF3 | TNF receptor associated fac... | NM_001199427.2 | 85.3% | 85.2% | 14A>G;568_569ins249 |
| 10 | human | 7187 | TRAF3 | TNF receptor associated fac... | XM_011537118.3 | 85.3% | 85.2% | 14A>G;568_569ins249 |
| 11 | human | 7187 | TRAF3 | TNF receptor associated fac... | XM_017021620.2 | 50.4% | 48.7% | (many diffs) |
| 12 | mouse | 22031 | Traf3 | TNF receptor-associated fac... | NM_011632.3 | 88.8% | 95.9% | (many diffs) |
| 13 | mouse | 22031 | Traf3 | TNF receptor-associated fac... | XM_006515798.2 | 88.8% | 95.9% | (many diffs) |
| 14 | mouse | 22031 | Traf3 | TNF receptor-associated fac... | XM_006515799.1 | 88.8% | 95.9% | (many diffs) |
| 15 | mouse | 22031 | Traf3 | TNF receptor-associated fac... | XM_006515800.2 | 88.8% | 95.9% | (many diffs) |
| 16 | mouse | 22031 | Traf3 | TNF receptor-associated fac... | XM_006515801.2 | 88.8% | 95.9% | (many diffs) |
| 17 | mouse | 22031 | Traf3 | TNF receptor-associated fac... | NM_001286122.1 | 84.5% | 91.5% | (many diffs) |
| 18 | mouse | 22031 | Traf3 | TNF receptor-associated fac... | XM_006515802.2 | 84.5% | 91.5% | (many diffs) |
| 19 | mouse | 22031 | Traf3 | TNF receptor-associated fac... | XM_017315042.1 | 84.5% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1773
- ORF length:
- 1704
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagtcgagt agaaagatgg actctcctgg cgcgctgcag actaacccgc 121 cgctaaagct gcacactgac cgcagtgctg ggacgccagt ttttgtccct gaacaaggag 181 gttacaagga aaagtttgtg aagaccgtgg aggacaagta caagtgtgag aagtgccacc 241 tggtgctgtg cagcccgaag cagaccgagt gtgggcaccg cttctgcgag agctgcatgg 301 cggccctgct gagctcttca agtccaaaat gtacagcgtg tcaagagagc atcgttaaag 361 ataaggtgtt taaggataat tgctgcaaga gagaaattct ggctcttcag atctattgtc 421 ggaatgaaag cagaggttgt gcagagcagt taatgctggg acatctgctg gtgcatttaa 481 aaaatgattg ccattttgaa gaacttccat gtgtgcgtcc tgactgcaaa gaaaaggtct 541 tgaggaaaga cctgcgagac cacgtggaga aggcgtgtaa ataccgggaa gccacatgca 601 gccactgcaa gagtcaggtt ccgatgatcg cgctgcagaa acacgaagac accgactgtc 661 cctgcgtggt ggtgtcctgc cctcacaagt gcagcgtcca gactctcctg aggagcgagt 721 tgagtgcaca cttgtcagag tgtgtcaatg cccccagcac ctgtagtttt aagcgctatg 781 gctgcgtttt tcaggggaca aaccagcaga tcaaggccca cgaggccagc tccgccgtgc 841 agcacgtcaa cctgctgaag gagtggagca actcgctcga aaagaaggtt tccttgttgc 901 agaatgaaag tgtagaaaaa aacaagagca tacaaagttt gcacaatcag atatgtagct 961 ttgaaattga aattgagaga caaaaggaaa tgcttcgaaa taatgaatcc aaaatccttc 1021 atttacagcg agtgatagac agccaagcag agaaactgaa ggagcttgac aaggagatcc 1081 ggcccttccg gcagaactgg gaggaagcag acagcatgaa gagcagcgtg gagtccctcc 1141 agaaccgcgt gaccgagctg gagagcgtgg acaagagcgc ggggcaagtg gctcggaaca 1201 caggcctgct ggagtcccag ctgagccggc atgaccagat gctgagtgtg cacgacatcc 1261 gcctagccga catggacctg cgcttccagg tcctggagac cgccagctac aatggagtgc 1321 tcatctggaa gattcgcgac tacaagcggc ggaagcagga ggccgtcatg gggaagaccc 1381 tgtcccttta cagccagcct ttctacactg gttactttGG CTATAAGATG TGTGCCAGGG 1441 TCTACCTGAA CGGGGACGGG ATGGGGAAGG GGACGCACTT GTCGCTGTTT TTTGTCATCA 1501 TGCGTGGAGA ATATGATGCC CTGCTTCCTT GGCCGTTTAA GCAGAAAGTG ACACTCATGC 1561 TGATGGATCA GGGGTCCTCT CGACGTCATT TGGGAGATGC ATTCAAGCCC GACCCCAACA 1621 GCAGCAGCTT CAAGAAGCCC ACTGGAGAGA TGAATATCGC CTCTGGCTGC CCAGTCTTTG 1681 TGGCCCAAAC TGTTCTAGAA AATGGGACAT ATATTAAAGA TGATACAATT TTTATTAAAG 1741 TCATAGTGGA TACTTCGGAT CTGCCCGATC CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1801 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1861 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 1921 AACCACCACA TGATTTTCTT CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1981 ttgtgaaaga tt