Construct: ORF TRCN0000476386
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017874.1_s317c1
- Derived from:
- ccsbBroadEn_11659
- DNA Barcode:
- TCAAGCAAACGCGAACTTATGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXO21 (23014)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476386
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23014 | FBXO21 | F-box protein 21 | XM_017019038.2 | 84.9% | 85% | 1_252del;1560A>G |
| 2 | human | 23014 | FBXO21 | F-box protein 21 | XM_017019037.2 | 83.9% | 83.9% | 1_252del;1143_1163del;1581A>G |
| 3 | human | 23014 | FBXO21 | F-box protein 21 | NM_015002.3 | 76.7% | 76.8% | 1_252del;1013_1192del;1740A>G |
| 4 | human | 23014 | FBXO21 | F-box protein 21 | XM_017019039.2 | 75.9% | 75.9% | 0_1ins207;554_733del;1281A>G |
| 5 | human | 23014 | FBXO21 | F-box protein 21 | NM_033624.3 | 75.9% | 75.9% | (many diffs) |
| 6 | mouse | 231670 | Fbxo21 | F-box protein 21 | NM_001346588.1 | 67.4% | 74.2% | (many diffs) |
| 7 | mouse | 231670 | Fbxo21 | F-box protein 21 | NM_145564.4 | 66.6% | 73.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1500
- ORF length:
- 1431
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaaacactac agccccaccg actacgtcaa ttggttggaa gagtataaag 121 ttcggcaaaa agctgggtta gaagcgcgga agattgtagc ctcgttctca aagaggttct 181 tttcagagca cgttccttgt aatggcttca gtgacattga gaaccttgaa ggaccagaga 241 ttttttttga ggatgaactg gtgtgtatcc taaatatgga aggaagaaaa gctttgacct 301 ggaaatacta cgcaaaaaaa attctttact acctgcggca acagaagatc ttaaataatc 361 ttaaggcctt tcttcagcag ccagatgact atgagtcgta tcttgaaggt gctgtatata 421 ttgaccagta ctgcaatcct ctctccgaca tcagcctcaa agacatccag gcccaaattg 481 acagcatcgt ggagcttgtt tgcaaaaccc ttcggggcat aaacagtcgc caccccagct 541 tggccttcaa ggcaggtgaa tcatccatga taatggaaat agaactccag agccaggtgc 601 tggatgccat gaactatgtc ctttacgacc aactgaagtt caaggggaat cgaatggatt 661 actataatgc cctcaactta tatatgcatc aggttttgat tcgcagaaca ggaatcccaa 721 tcagcatgtc tctgctctat ttgacaattg ctcggcagtt gggagtccca ctggagcctg 781 tcaacttccc aagtcacttc ttattaaggt ggtgccaagg cgcagaaggg gaaggcatcg 841 accagtcata ccagctcctg agagactcgc tggatctcta tctggcaatg tacccggacc 901 aggtgcagct tctcctcctc caagccaggc tttacttcca cctgggaatc tggccagaga 961 aggtgcttga catcctccag cacatccaaa ccctagaccc ggggcagcac ggggcggtgg 1021 gctacctggt gcagcacact ctagagcaca ttgagcgcaa aaaggaggag gtgggcgtag 1081 aggtgaagct gcgctccgat gagaagcaca gagatgtctg ctactccatc gggctcatta 1141 tgaagcataa gaggtatggc tataactgtg tgatctacgg ctgggacccc accTGCATGA 1201 TGGGACACGA GTGGATCCGG AACATGAACG TCCACAGCCT GCCGCACGGC CACCACCAGC 1261 CTTTCTATAA CGTGCTGGTG GAGGACGGCT CCTGTCGATA CGCAGCCCAA GAAAACTTGG 1321 AATATAACGT GGAGCCTCAA GAAATCTCAC ACCCTGACGT GGGACGCTAT TTCTCGGAGT 1381 TTACTGGCAC TCACTACATC CCAAACGCAG AGCTGGAGAT CCGGTATCCA GAAGATCTGG 1441 AGTTTGTCTA TGAAACGGTG CAGAATATTT ACAGTGCAAA GAAAGAGAAC ATAGATGAGT 1501 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1561 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1621 TTTATATATC TTGTGGAAAG GACGATCAAG CAAACGCGAA CTTATGCCGA CGCGTTAAGT 1681 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt