Construct: ORF TRCN0000476392
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007555.1_s317c1
- Derived from:
- ccsbBroadEn_10656
- DNA Barcode:
- CATTCTCCCAGAGTAAGGCATTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MGAT4EP (641515)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476392
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 641515 | MGAT4EP | MGAT4 family member E, pseu... | NR_038135.2 | 21.7% | 1_1100del;1253T>C;1716_2819del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 684
- ORF length:
- 615
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagctttgcc acaaagttct ctgattactt cctgttaata gaggacaatg 121 tcttttttgc ccccaacttt gtcacccaca ttcattcaag ggtgaccacc atgaagtcca 181 actcgtgggt gctaatggag ttctccaata tgggcttcct cggcaaactc ttccacagca 241 gggacctccc actcctggcc catttcctcc ttctcttcta caaggagaaa ccccttgaca 301 gcctgatttc tcatttccat acgctcctgg tccaggaaag cccaatcctc tgcagacctt 361 tcctattcta ctgcagggtc tcctacactg cctttgatca caagcagaag gccacagcac 421 ttcagaaaaa gaatgccgat ggtcccaaca acccgcctGG AGCTGTCTTC ACTGATATGC 481 AGGTTTCCGA TGTGCATTTC CCCTGGGAGG CCTACACTCT GGATGAGTCA TTCTTTTGGA 541 CACACAACAT TAGTATAGGA AACCACCTGA CAGTGATTCT GAACCATCCA GCAAACCTGA 601 GCAGGGTGCA AGTGATGACG GGCTCCATTG TGGAATGGGA GGTACGCCCT GGAGAAGGGG 661 CAGGTGGAGC TGGGCTACCA ACCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 721 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 781 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC ATTCTCCCAG 841 AGTAAGGCAT TTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 901 att