Construct: ORF TRCN0000476403
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010214.1_s317c1
- Derived from:
- ccsbBroadEn_00383
- DNA Barcode:
- GGCCAAACATTTGCCCGCCGATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A10 (1468)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476403
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1468 | SLC25A10 | solute carrier family 25 me... | NM_012140.5 | 100% | 100% | |
| 2 | human | 1468 | SLC25A10 | solute carrier family 25 me... | NM_001270888.2 | 96.9% | 96.9% | 628_654del |
| 3 | human | 1468 | SLC25A10 | solute carrier family 25 me... | XM_017024220.1 | 85% | 85% | 0_1ins129 |
| 4 | human | 1468 | SLC25A10 | solute carrier family 25 me... | NM_001270953.1 | 59.8% | 44.3% | 529_530ins83;779_1218del |
| 5 | mouse | 27376 | Slc25a10 | solute carrier family 25 (m... | NM_013770.2 | 85.9% | 89.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 927
- ORF length:
- 861
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agccgaggcg cgcgtgtcgc gctggtactt cggggggctg gcctcctgcg 121 gggccgcctg ctgcacgcac ccgctggacc tgctcaaggt gcatctgcag acgcagcagg 181 aggtgaagct gcgcatgacg ggcatggcgc tgcgggtggt gcgtaccgac ggcatcctgg 241 cactctacag cggcctgagc gcctcgctgt gcagacagat gacctactcc ctgactcggt 301 tcgccatcta cgagactgtg cgggaccgtg tggccaaggg cagccagggg cctctcccct 361 tccacgagaa ggtgttgctg ggctccgtca gcggtttagc tggaggcttc gtggggacgc 421 ccgcagactt ggtcaacgtc aggatgcaga acgacgtgaa gctgccccag ggtcagcggc 481 gcaactacgc ccatgcgctg gatggcctgt accgcgtagc tcgtgaagag ggtctcagga 541 gactgttctc gggtgcaacc atggcatcca gccgaggggc cttagtcact gtgggccagc 601 tgtccTGCTA CGACCAGGCC AAGCAGCTGG TCCTTAGCAC CGGGTACCTC TCTGACAACA 661 TCTTCACTCA CTTTGTCGCC AGCTTTATTG CAGGTGGATG TGCCACGTTC CTGTGCCAGC 721 CCCTGGATGT GCTGAAGACT CGCCTGATGA ACTCCAAGGG GGAGTATCAG GGCGTTTTCC 781 ACTGCGCCGT GGAGACAGCG AAGCTCGGGC CTCTGGCCTT TTACAAGGGC CTCGTCCCAG 841 CTGGCATCCG CCTCATCCCC CACACCGTGC TCACTTTTGT GTTTCTGGAA CAGCTACGCA 901 AAAACTTTGG CATCAAAGTG CCATCCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 961 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1021 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGCCAAAC 1081 ATTTGCCCGC CGATTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1141 aagatt