Construct: ORF TRCN0000476460
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009911.1_s317c1
- Derived from:
- ccsbBroadEn_07380
- DNA Barcode:
- GCCAATGTTAGAGGTTTTTAATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPOAP1 (9256)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476460
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9256 | TSPOAP1 | TSPO associated protein 1 | NM_004758.4 | 99.9% | 99.9% | 4344G>A;5361G>T |
| 2 | human | 9256 | TSPOAP1 | TSPO associated protein 1 | NM_001261835.2 | 99.4% | 99.4% | 4344G>A;5149_5150ins27;5334G>T |
| 3 | human | 9256 | TSPOAP1 | TSPO associated protein 1 | NM_024418.3 | 96.7% | 96.7% | 569_570ins180;4164G>A;5181G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 5640
- ORF length:
- 5571
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcaactg acaaccctcc cacggcctgg ggaccctgga gccatggagc 121 catgggcact gcccacctgg catagctgga ctccaggtcg agggggtgaa cctagcagtg 181 cagccccaag catcgctgat actcctccgg cagctctgca gcttcaagaa ctgaggtctg 241 aggagagttc caagcccaaa ggagacggga gctccaggcc cgtgggggga actgaccctg 301 aaggagcaga ggcttgtctg cccagcctgg gccagcaagc atccagctct ggacccgcct 361 gccagaggcc agaggatgag gaagtggagg ctttcctgaa ggccaagctg aatatgagct 421 ttggggacag gcccaatctg gagctgctga gggccctggg ggagctgcgg cagcgctgtg 481 ccatccttaa ggaggaaaac cagatgctga ggaagagcag cttccctgag acagaagaga 541 aggtgcggag gctgaagagg aagaacgccg agctggcggt cattgccaag cgcctggagg 601 agagggcccg aaagctgcag gaaacgaacc tgagggtggt gagtgccccc ttgccccggc 661 cggggaccag cttggagttg tgtcggaagg ccctagcccg ccagcgagcc cgggacctca 721 gtgagacagc cagtgcactg ctggccaagg acaagcagat tgctgccttg cagcgggagt 781 gcagggagct gcaggccagg ctcactctgg tgggcaagga gggtccccag tggctccacg 841 tgcgggactt cgatcggctg ctgcgcgagt cccagcggga ggtgctgcgg ctgcagaggc 901 agatcgcgct gcgcaaccag cgggagacgc tcccgctccc gccgtcctgg cccccgggcc 961 ctgctctcca ggccagagca ggggcgcctg ctcccggggc cccgggagag gccacgcccc 1021 aggaggatgc ggacaaccta cccgtgattc taggggagcc agagaaagag cagagggtgc 1081 agcagctgga atcggagctc agcaagaagc ggaagaaatg cgagagcctg gagcaggaag 1141 cccggaaaaa gcagaggcga tgtgaggagc tggaactgca gctgagacaa gcgcagaatg 1201 agaatgcccg cctggtggag gagaactccc ggctcagtgg gagagccaca gagaaggagc 1261 aggtggagtg ggagaatgcg gagctgaggg gccagctcct gggggtgaca caggagaggg 1321 actcagccct tcgcaagagc cagggcctgc agagcaagct ggagagcctg gagcaagtgc 1381 tgaagcacat gcgggaggtg gcccagcggc ggcagcagct ggaggtggag catgaacagg 1441 ctcggctcag cctacgggag aagcaggagg aggtccggag actgcagcag gcccaggctg 1501 aagcccagag ggaacatgaa ggagccgtgc agctgctgga gtctaccttg gattccatgc 1561 aggcccgggt tcgagagctc gaagaacagt gccgcagcca aaccgagcag ttcagcctcc 1621 tggcacagga actccaggct ttccgcctgc acccgggccc cttggatctg ctcacatctg 1681 ccctggactg tgggagcctt ggagactgcc caccaccccc ctgctgctgc tccattcccc 1741 agccttgccg ggggtctggc cccaaagacc ttgacctccc gccgggctcc cctgggcgct 1801 gcaccccaaa gtcttccgag cctgcccctg ccactctcac tggggtccct cgaaggacag 1861 ccaagaaggc agagtctctc tccaactcct cccactccga gtccatccac aacagcccca 1921 agtcatgccc tacacctgag gtggacacag ccagtgaggt agaggagctg gaggcagaca 1981 gtgtctccct gctcccagct gcgccagagg gcagccgggg aggagccagg atccaggtct 2041 tcctagcacg ttatagctac aacccctttg agggtcccaa tgagaatcca gaagcagagc 2101 ttccgctgac agctggcgag tacatctaca tctatggcaa catggatgag gatggctttt 2161 ttgaaggaga gctcatggat ggccgaaggg gcctggtccc ttccaatttt gtagagcgtg 2221 tgtcggatga tgacctcctg acctccctcc ctccagagct ggccgatttg tcccacagct 2281 caggccctga actcagtttc ctgagtgtag gtgggggtgg cagcagtagc gggggccaaa 2341 gcagtgtggg aaggagccag cccagacctg aggaggagga tgcaggggac gagctcagtc 2401 tgagcccatc accggagggc ctgggcgagc ctcctgccgt gccttacccc cgccgtctgg 2461 tggtcctcaa gcagctggcc cacagcgtgg tgctggcctg ggagccgcct cctgagcaag 2521 tggagctaca cggcttccat atctgtgtga atggggagct gcgacaggcc ctggggcctg 2581 gggcgccacc caaggccgtg ctggagaacc tggacctgtg ggccgggccc cttcacattt 2641 ctgtccaggc cctgactagc cggggcagct ctgacccact gcgctgttgc ttggcggtgg 2701 gtgcccgggc cggagtggtg cccagccagc tgcgggtcca tcggttgaca gccacatctg 2761 ctgagatcac ctgggtgccc ggcaatagca acttggccca tgccatctac ctcaatgggg 2821 aagagtgccc acctgccagc cccagtacct actgggccac cttctgccac ttacggcctg 2881 gcacacccta tcaggcccaa gtggaggctc agctcccacc ccaagggccc tgggaaccag 2941 gctgggagag gctggagcag cgggctgcca ccctgcagtt caccacactc ccagcaggcc 3001 cacctgatgc ccctctggat gtgcagatcg agcctgggcc ctcccctggg atcttgatca 3061 tcagttggct cccagtcacc atcgatgctg ctggcacatc caacggtgtc cgggtcacag 3121 gctatgccat ctacgctgat gggcagaaga tcatggaggt ggcctcaccc acggcaggca 3181 gtgtactggt ggagttgtcc cagctgcagc tgctgcaggt gtgtcgtgag gtggtcgtgc 3241 gcaccatgtc gccccacggg gagtcggcgg actccatccc ggctcctatc actcccgccc 3301 tggctccggc cagcctgcca gcccgagtct cctgcccctc accgcaccca agcccagagg 3361 ccagagcgcc ccttgcttca gcctccccag ggcctggaga ccccagctct cctctccagc 3421 accctgctcc ccttggaact caagagcctc caggagcacc ccctgcaagc ccttccagag 3481 agatggcaaa agggtcccac gaggaccctc cagcaccttg ctcccaggag gaggctgggg 3541 cagcagtgct gggcacctca gaggagagga cagccagcac atctaccctg ggtgagaagg 3601 accctggccc cgcagctccc tcactggcca agcaggaggc cgagtggact gcaggagagg 3661 cctgtccggc ctccagctcc acccagggag cacgggccca gcaggcgcca aataccgaga 3721 tgtgccaagg aggagaccca gggtctgggc tgaggcccag ggctgagaag gaggacacag 3781 cagagcttgg ggttcatctg gtgaactccc tcgtggacca cggccgcaac tcagacctgt 3841 cagacatcca ggaggaagag gaagaggagg aggaggagga ggaagaggag ctgggttcca 3901 ggacttgctc cttccagaag caggttgctg gcaacagcat cagggagaat ggggccaagt 3961 cccagcccga ccccttttgt gagactgaca gcgatgagga gatcttggag cagatcctgg 4021 agctgcccct ccagcagttc tgtagcaaga agctctttag catcccggag gaggaggaag 4081 aggaagagga ggacgaggag gaggagaagt caggggcagg ctgttcttcc cgagaccctg 4141 gcccgcctga acctgcattg ctggggctgg gctgtgacag tggtcagccc cgaagacctg 4201 gccagtgtcc cttgtctcct gagtcctcca gggctggaga ctgcctggaa gacatgcctg 4261 gattagttgg tggaagcagc cggaggagag gagggggctc ccctgagaag cccccaagcc 4321 gcaggcggcc tccagatccc cgagaacact gcagccgact tctcagcaac aatgggcccc 4381 aggcctctgg acgactgggc cccacacggg aaaggggtgg cctccccgta attgagggcc 4441 ccaggactgg actagaggct agcgggagag gccggctggg cccttcccgg aggtgctccc 4501 gtggccgggc gctggagcct ggcctggcca gctgcctttc ccccaagtgc ttggaaatca 4561 gcattgaata tgattcggag gatgagcagg aggcgggcag cgggggcatc agcatcacca 4621 gctcctgcta ccctggagat ggggaggcct ggggcacagc aactgtagga aggcccaggg 4681 ggcctccgaa ggccaattca ggccccaagc cctacccacg cctcccagcc tgggagaaag 4741 gggaaccaga gcggagaggc cgcagtgcga cgggcagagc caaggagcca ctctcccggg 4801 caacagagac cggagaggcc agagggcagg acggctctgg gcggaggggc ccccagaaga 4861 gaggtgtccg agtcctcagg ccaagcactg cagagctagt ccctgcgagg agcccctcag 4921 aaacactggc ttaccagcac ctacccgtca ggatctttgt ggctctgttt gactatgacc 4981 ccgtgtcaat gtcgcccaat cctgatgctg gagaagaaga gcttcccttc cgagagggtc 5041 agatcctgaa ggtgtttggg gacaaggatg ccgatggctt ctaccagggc gaaggtgggg 5101 gccggacagg ctacattccc tgcaacatgg tggctgaggt ggctgtggac agccctgctg 5161 ggagacagca actgctccag cggggttatt tgtccccaga tattctcctt gagggctcag 5221 ggaatggtcc gtttgtgtac tccacagccc acacaactgg gCCTCCTCCC AAGCCCCGCC 5281 GCTCCAAGAA AGCTGAGTCG GAAGGCCCTG CCCAGCCCTG TCCAGGCCCC CCTAAGCTGG 5341 TCCCCTCTGC TGACCTGAAA GCTCCCCACT CCATGGTGGC TGCATTTGAC TACAACCCCC 5401 AGGAGAGTTC CCCCAATATG GACGTGGATG CAGAGCTGCC CTTCCGGGCA GGGGATGTCA 5461 TTACTGTGTT TGGGGGCATG GACGATGACG GTTTCTACTA TGGGGAATTA AATGGACAAA 5521 GGGGCCTGGT TCCATCCAAC TTCCTGGAGG GCCCTGGGCC TGAGGCAGGC GGCCTGGACA 5581 GGGAACCCAG GACACCCCAG GCTGAGAGTC AGAGAACGAG GAGGAGAAGA GTCCAGTGCT 5641 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 5701 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 5761 TTTATATATC TTGTGGAAAG GACGAGCCAA TGTTAGAGGT TTTTAATTCA CGCGTTAAGT 5821 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt