Construct: ORF TRCN0000476464
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011222.1_s317c1
- Derived from:
- ccsbBroadEn_06739
- DNA Barcode:
- CCCAATACAGCAAACTTAGTACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLOD1 (5351)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476464
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5351 | PLOD1 | procollagen-lysine,2-oxoglu... | NM_000302.4 | 99.8% | 99.7% | 295G>A;358G>T;1632A>C |
| 2 | human | 5351 | PLOD1 | procollagen-lysine,2-oxoglu... | NM_001316320.2 | 93.7% | 93.6% | (many diffs) |
| 3 | mouse | 18822 | Plod1 | procollagen-lysine, 2-oxogl... | NM_011122.3 | 86% | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2247
- ORF length:
- 2181
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gcccctgctg ctactggccc tgctgggctg gctgctgctg gccgaagcga 121 agggcgacgc caagccggag gacaaccttt tagtcctcac ggtggccact aaggagaccg 181 agggattccg tcgcttcaag cgctcagctc agttcttcaa ctacaagatc caggcgcttg 241 gcctagggga ggactggaat gtggagaagg ggacgtcggc aggtggaggg cagaaggtcc 301 ggctgctgaa gaaagctctg gagaagcacg cagacaagga ggatctggtc attctcttca 361 cagacagcta tgacgtgctg tttgcatcgg ggccccggga gctcctgaag aagttccggc 421 agtccaggag ccaggtggtc ttctctgctg aggagctcat ctacccagac cgcaggctgg 481 agaccaagta tccggtggtg tccgatggca agaggttcct gggctctgga ggcttcatcg 541 gttatgcccc caacctcagc aaactggtgg ccgagtggga gggccaggac agcgacagcg 601 atcagctgtt ttacaccaag atcttcttgg acccggagaa gagggagcag atcaatatca 661 ccctggacca ccgctgccgt atcttccaga acctggatgg agccttggat gaggtcgtgc 721 tcaagtttga aatgggccat gtgagagcga ggaacctggc ctatgacacc ctcccggtcc 781 tgatccatgg caacgggcca accaagctgc agttgaacta cctgggcaac tacatcccgc 841 gcttctggac cttcgaaaca ggctgcaccg tgtgtgacga aggcttgcgc agcctcaagg 901 gcattgggga tgaagctctg cccacggtcc tggtcggcgt gttcatcgaa cagcccacgc 961 cgtttgtgtc cctgttcttc cagcggctcc tgcggctcca ctacccccag aaacacatgc 1021 gacttttcat ccacaaccac gagcagcacc acaaggctca ggtggaagag ttcctggcac 1081 agcatggcag cgagtaccag tctgtgaagc tggtgggccc tgaggtgcgg atggcgaatg 1141 cagatgccag gaacatgggc gcagacctgt gccggcagga ccgcagctgc acctactact 1201 tcagcgtgga tgctgacgtg gccctgaccg agcccaacag cctgcggctg ctgatccaac 1261 agaacaagaa cgtcattgcc ccgctgatga cccggcatgg gaggctgtgg tcgaacttct 1321 ggggggctct cagtgcagat ggctactatg cccgttccga ggactacgtg gacattgtgc 1381 aggggcggcg tgttggtgtc tggaatgtgc cctatatttc aaacatctac ttgatcaagg 1441 gcagtgccct gcggggtgag ctgcagtcct cagatctctt ccaccacagc aagctggacc 1501 ccgacatggc cttctgtgcc aacatccggc agcaggatgt gttcatgttc ctgaccaacc 1561 ggcacaccct tggccatctg ctctccctag acagctaccg caccacccac ctgcacaacg 1621 acctctggga ggtgttcagc aaccccgagg actggaagga gaagtacatc caccagaact 1681 acaccaaagc cctggccggg aagctggtgg agacgccctg cccggatgtc tattggttcc 1741 ccatcttcac ggaggtggcc tgtgatgagc tggtggagga gatggagcac tttggccagt 1801 ggtctctggg caacaacaag gacaaccgca tccagggtgg ctacgagaac gtgccgacta 1861 ttgacatcca catgaaccag atcggctttg agcgggagtg gcacaaattc ctgctggagt 1921 acattgcgcc catgacggag aagctctacc ccggctacta caccagggcc cagtttgacc 1981 tggcctttgt cgtccgctac aagccTGATG AGCAGCCCTC ACTGATGCCA CACCATGATG 2041 CCTCCACCTT CACCATCAAC ATCGCCCTGA ACCGAGTCGG GGTGGATTAC GAGGGCGGGG 2101 GCTGTCGGTT CCTGCGCTAC AACTGTTCCA TCCGAGCCCC AAGGAAGGGC TGGACCCTCA 2161 TGCACCCTGG ACGACTCACG CATTACCATG AGGGGCTCCC CACCACCAGG GGCACCCGCT 2221 ACATCGCAGT CTCCTTCGTC GATCCCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 2281 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2341 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCCAATAC 2401 AGCAAACTTA GTACCCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2461 aagatt