Construct: ORF ccsbBroadEn_06739

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011222.1_s300c1, BRDN0000457116
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PLOD1 (5351)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5351 PLOD1 procollagen-lysine,2-oxoglu... NM_000302.4 99.8% 99.7% 295G>A;358G>T;1632A>C
2 human 5351 PLOD1 procollagen-lysine,2-oxoglu... NM_001316320.2 93.7% 93.6% (many diffs)
3 mouse 18822 Plod1 procollagen-lysine, 2-oxogl... NM_011122.3 86% 91.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2247
ORF length:
2181
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCG GCCCCTGCTG CTACTGGCCC TGCTGGGCTG GCTGCTGCTG GCCGAAGCGA
121AGGGCGACGC CAAGCCGGAG GACAACCTTT TAGTCCTCAC GGTGGCCACT AAGGAGACCG
181AGGGATTCCG TCGCTTCAAG CGCTCAGCTC AGTTCTTCAA CTACAAGATC CAGGCGCTTG
241GCCTAGGGGA GGACTGGAAT GTGGAGAAGG GGACGTCGGC AGGTGGAGGG CAGAAGGTCC
301GGCTGCTGAA GAAAGCTCTG GAGAAGCACG CAGACAAGGA GGATCTGGTC ATTCTCTTCA
361CAGACAGCTA TGACGTGCTG TTTGCATCGG GGCCCCGGGA GCTCCTGAAG AAGTTCCGGC
421AGTCCAGGAG CCAGGTGGTC TTCTCTGCTG AGGAGCTCAT CTACCCAGAC CGCAGGCTGG
481AGACCAAGTA TCCGGTGGTG TCCGATGGCA AGAGGTTCCT GGGCTCTGGA GGCTTCATCG
541GTTATGCCCC CAACCTCAGC AAACTGGTGG CCGAGTGGGA GGGCCAGGAC AGCGACAGCG
601ATCAGCTGTT TTACACCAAG ATCTTCTTGG ACCCGGAGAA GAGGGAGCAG ATCAATATCA
661CCCTGGACCA CCGCTGCCGT ATCTTCCAGA ACCTGGATGG AGCCTTGGAT GAGGTCGTGC
721TCAAGTTTGA AATGGGCCAT GTGAGAGCGA GGAACCTGGC CTATGACACC CTCCCGGTCC
781TGATCCATGG CAACGGGCCA ACCAAGCTGC AGTTGAACTA CCTGGGCAAC TACATCCCGC
841GCTTCTGGAC CTTCGAAACA GGCTGCACCG TGTGTGACGA AGGCTTGCGC AGCCTCAAGG
901GCATTGGGGA TGAAGCTCTG CCCACGGTCC TGGTCGGCGT GTTCATCGAA CAGCCCACGC
961CGTTTGTGTC CCTGTTCTTC CAGCGGCTCC TGCGGCTCCA CTACCCCCAG AAACACATGC
1021GACTTTTCAT CCACAACCAC GAGCAGCACC ACAAGGCTCA GGTGGAAGAG TTCCTGGCAC
1081AGCATGGCAG CGAGTACCAG TCTGTGAAGC TGGTGGGCCC TGAGGTGCGG ATGGCGAATG
1141CAGATGCCAG GAACATGGGC GCAGACCTGT GCCGGCAGGA CCGCAGCTGC ACCTACTACT
1201TCAGCGTGGA TGCTGACGTG GCCCTGACCG AGCCCAACAG CCTGCGGCTG CTGATCCAAC
1261AGAACAAGAA CGTCATTGCC CCGCTGATGA CCCGGCATGG GAGGCTGTGG TCGAACTTCT
1321GGGGGGCTCT CAGTGCAGAT GGCTACTATG CCCGTTCCGA GGACTACGTG GACATTGTGC
1381AGGGGCGGCG TGTTGGTGTC TGGAATGTGC CCTATATTTC AAACATCTAC TTGATCAAGG
1441GCAGTGCCCT GCGGGGTGAG CTGCAGTCCT CAGATCTCTT CCACCACAGC AAGCTGGACC
1501CCGACATGGC CTTCTGTGCC AACATCCGGC AGCAGGATGT GTTCATGTTC CTGACCAACC
1561GGCACACCCT TGGCCATCTG CTCTCCCTAG ACAGCTACCG CACCACCCAC CTGCACAACG
1621ACCTCTGGGA GGTGTTCAGC AACCCCGAGG ACTGGAAGGA GAAGTACATC CACCAGAACT
1681ACACCAAAGC CCTGGCCGGG AAGCTGGTGG AGACGCCCTG CCCGGATGTC TATTGGTTCC
1741CCATCTTCAC GGAGGTGGCC TGTGATGAGC TGGTGGAGGA GATGGAGCAC TTTGGCCAGT
1801GGTCTCTGGG CAACAACAAG GACAACCGCA TCCAGGGTGG CTACGAGAAC GTGCCGACTA
1861TTGACATCCA CATGAACCAG ATCGGCTTTG AGCGGGAGTG GCACAAATTC CTGCTGGAGT
1921ACATTGCGCC CATGACGGAG AAGCTCTACC CCGGCTACTA CACCAGGGCC CAGTTTGACC
1981TGGCCTTTGT CGTCCGCTAC AAGCCTGATG AGCAGCCCTC ACTGATGCCA CACCATGATG
2041CCTCCACCTT CACCATCAAC ATCGCCCTGA ACCGAGTCGG GGTGGATTAC GAGGGCGGGG
2101GCTGTCGGTT CCTGCGCTAC AACTGTTCCA TCCGAGCCCC AAGGAAGGGC TGGACCCTCA
2161TGCACCCTGG ACGACTCACG CATTACCATG AGGGGCTCCC CACCACCAGG GGCACCCGCT
2221ACATCGCAGT CTCCTTCGTC GATCCCTACC CAACTTTCTT GTACAAAGTT GGcattataa
2281gaaagcattg cttatcaatt tgttgcaacg aac

Download FASTA (ORF) (Full)