Construct: ORF TRCN0000476490
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000741.1_s317c1
- Derived from:
- ccsbBroadEn_06106
- DNA Barcode:
- CAGTGACTTATCGTATTTGTCTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNM1 (1759)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476490
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1759 | DNM1 | dynamin 1 | NM_001005336.3 | 99.9% | 99.8% | 1008C>T;1666G>T |
| 2 | human | 1759 | DNM1 | dynamin 1 | NM_001288737.2 | 98.4% | 98.1% | (many diffs) |
| 3 | human | 1759 | DNM1 | dynamin 1 | NM_001288738.2 | 98.4% | 98.1% | (many diffs) |
| 4 | human | 1759 | DNM1 | dynamin 1 | NM_004408.4 | 97.9% | 97.8% | (many diffs) |
| 5 | human | 1759 | DNM1 | dynamin 1 | NM_001374269.1 | 97.9% | 97.4% | (many diffs) |
| 6 | human | 1759 | DNM1 | dynamin 1 | NM_001288739.2 | 96.5% | 96% | (many diffs) |
| 7 | mouse | 13429 | Dnm1 | dynamin 1 | XM_006497661.2 | 92.5% | 99.5% | (many diffs) |
| 8 | mouse | 13429 | Dnm1 | dynamin 1 | XM_017315328.1 | 92.5% | 99.5% | (many diffs) |
| 9 | mouse | 13429 | Dnm1 | dynamin 1 | NM_001301737.1 | 91.2% | 97.8% | (many diffs) |
| 10 | mouse | 13429 | Dnm1 | dynamin 1 | XM_006497658.3 | 91.2% | 97.8% | (many diffs) |
| 11 | mouse | 13429 | Dnm1 | dynamin 1 | XM_006497659.3 | 91.2% | 97.8% | (many diffs) |
| 12 | mouse | 13429 | Dnm1 | dynamin 1 | XM_006497660.2 | 91.2% | 97.8% | (many diffs) |
| 13 | mouse | 13429 | Dnm1 | dynamin 1 | XM_006497655.2 | 90.7% | 97.4% | (many diffs) |
| 14 | mouse | 13429 | Dnm1 | dynamin 1 | XM_006497650.2 | 90.6% | 97% | (many diffs) |
| 15 | mouse | 13429 | Dnm1 | dynamin 1 | NM_010065.3 | 90.1% | 96.5% | (many diffs) |
| 16 | mouse | 13429 | Dnm1 | dynamin 1 | XM_006497654.3 | 89.4% | 95.8% | (many diffs) |
| 17 | mouse | 13429 | Dnm1 | dynamin 1 | XM_006497651.2 | 89.3% | 95.5% | (many diffs) |
| 18 | mouse | 13429 | Dnm1 | dynamin 1 | XM_006497649.2 | 89.2% | 95.3% | (many diffs) |
| 19 | mouse | 13429 | Dnm1 | dynamin 1 | XM_006497652.2 | 89.2% | 95.3% | (many diffs) |
| 20 | mouse | 13429 | Dnm1 | dynamin 1 | XM_006497653.2 | 88.8% | 94.9% | (many diffs) |
| 21 | mouse | 13429 | Dnm1 | dynamin 1 | XR_374057.2 | 83.5% | (many diffs) | |
| 22 | mouse | 13429 | Dnm1 | dynamin 1 | XM_006497662.2 | 82.8% | 86.5% | (many diffs) |
| 23 | mouse | 13429 | Dnm1 | dynamin 1 | XR_374058.3 | 70.4% | (many diffs) | |
| 24 | mouse | 13429 | Dnm1 | dynamin 1 | XR_001780678.1 | 69.9% | (many diffs) | |
| 25 | mouse | 13429 | Dnm1 | dynamin 1 | NR_125959.1 | 69.4% | (many diffs) | |
| 26 | mouse | 13429 | Dnm1 | dynamin 1 | XR_374055.2 | 60% | (many diffs) | |
| 27 | mouse | 13429 | Dnm1 | dynamin 1 | XR_001780675.1 | 59.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2622
- ORF length:
- 2553
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcaaccgc ggcatggaag atctcatccc gctggtcaac cggctgcaag 121 acgccttctc tgccatcggc cagaacgcgg acctcgacct gccgcagatc gctgtggtgg 181 gcggccagag cgccggcaag agctcggtgc tcgagaattt cgtaggcagg gacttcttgc 241 ctcgaggatc tggcattgtc acccgacgtc ccctggtctt gcagctggtc aatgcaacca 301 cagaatatgc cgagttcctg cactgcaagg gaaagaaatt caccgacttc gaggaggtgc 361 gccttgagat cgaggccgag accgacaggg tcaccggcac caacaagggc atctcgccgg 421 tgcctatcaa cctccgcgtc tactcgccgc acgtgctgaa cctgaccctg gtggacctgc 481 ccggaatgac caaggtcccg gtgggggacc aacctcccga catcgagttc cagatccgag 541 acatgcttat gcagtttgtc accaaggaga actgcctcat cctggccgtg tcccccgcca 601 actctgacct ggccaattct gacgccctca aggtcgccaa ggaggtggac ccccagggcc 661 agcgcaccat cggggtcatc accaagctgg acctgatgga cgagggcaca gatgcccgtg 721 atgtgctgga gaacaagctg ctccccctgc gcagaggcta cattggagtg gtgaaccgga 781 gccagaagga cattgatggc aagaaggaca ttaccgccgc cttggctgct gaacgaaagt 841 tcttcctctc ccatccatct tatcgccact tggctgaccg tatgggcacg ccctacctgc 901 agaaggtcct caatcagcaa ctgacgaacc acatccggga cacactgccg gggctgcgga 961 acaagctgca gagccagcta ctgtccattg agaaggaggt ggaggaatac aagaacttcc 1021 gccctgatga cccagctcgc aagaccaagg ccctgctgca gatggtccag cagtttgccg 1081 tagactttga gaagcgcatt gagggctcag gagatcagat cgacacctac gaactgtcag 1141 ggggagcccg cattaaccga atcttccacg agcgcttccc tttcgagctg gtcaagatgg 1201 agtttgatga gaaggaactc cgaagggaga tcagctatgc tatcaagaat atccatggca 1261 ttagaacggg gctgtttacc ccagacatgg cctttgagac cattgtgaaa aagcaggtga 1321 agaagatccg agaaccgtgt ctcaagtgtg tggacatggt tatctcggag ctaatcagca 1381 ccgttagaca gtgcaccaag aagctccagc agtacccgcg gctacgggag gagatggagc 1441 gcatcgtgac cacccacatc cgggagcgcg agggccgcac taaggagcag gtcatgcttc 1501 tcatcgatat cgagctggct tacatgaaca ccaaccatga ggacttcata ggctttgcca 1561 atgctcagca gaggagcaac cagatgaaca agaagaagac ttcagggaac caggatgaga 1621 ttctggtcat ccgcaagggc tggctgacta tcaataatat tggcatcatg aaagggggct 1681 ccaaggagta ctggtttgtg ctgactgctg agaatctgtc ctggtacaag gattatgagg 1741 agaaagagaa gaaatacatg ctgtctgtgg acaacctcaa gctgcgggac gtggagaagg 1801 gctttatgtc gagcaagcat atctttgccc tctttaacac ggagcagagg aatgtctaca 1861 aggattatcg gcagctggag ctagcctgtg agacacagga ggaggtggac agctggaagg 1921 cctccttcct gagggctggc gtgtaccctg agcgtgttgg ggacaaagag aaagccagcg 1981 agaccgagga gaatggctcc gacagcttca tgcattccat ggacccacag ctggaacggc 2041 aagtggagac catccggaat cttgtggact catacatggc cattgtcaac aagaccgtga 2101 gggacctcat gcccaagacc atcatgcacc tcatgattaa caataccaag gagttcatct 2161 tctcggagct gctggccaac ctgtactcgt gtggggacca gaacacgctg atggaggagt 2221 cggcggagca ggcacagcgg cgcgacgaga tgctgcgcat gtaccacgca ctgaaggagg 2281 cgctcagcaT CATCGGCGAC ATCAACACGA CCACCGTCAG CACGCCCATG CCCCCGCCCG 2341 TGGACGACTC CTGGCTGCAG GTGCAGAGCG TACCGGCCGG ACGCAGGTCG CCCACGTCCA 2401 GCCCCACGCC GCAGCGCCGA GCCCCCGCCG TGCCCCCAGC CCGGCCCGGG TCGCGGGGCC 2461 CTGCTCCTGG GCCTCCGCCT GCTGGGTCCG CCCTGGGGGG GGCGCCCCCC GTGCCCTCCA 2521 GGCCGGGGGC TTCCCCTGAC CCTTTCGGCC CTCCCCCTCA GGTGCCCTCG CGCCCCAACC 2581 GCGCCCCGCC CGGGGTCCCC AGAATCACTA TCAGTGACCC CTTGCCAACT TTCTTGTACA 2641 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2701 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2761 AGGACGACAG TGACTTATCG TATTTGTCTG AACGCGTTAA GTCgacaatc aacctctgga 2821 ttacaaaatt tgtgaaagat t