Construct: ORF TRCN0000476520
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000116.1_s317c1
- Derived from:
- ccsbBroadEn_15029
- DNA Barcode:
- ATCACTACACCCGGTGAGACATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYRIP (25924)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476520
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25924 | MYRIP | myosin VIIA and Rab interac... | NM_001284425.1 | 99.7% | 99.6% | (many diffs) |
| 2 | human | 25924 | MYRIP | myosin VIIA and Rab interac... | NM_001284423.1 | 89.4% | 82.4% | (many diffs) |
| 3 | human | 25924 | MYRIP | myosin VIIA and Rab interac... | NM_015460.4 | 89.4% | 82.4% | (many diffs) |
| 4 | human | 25924 | MYRIP | myosin VIIA and Rab interac... | XM_011533575.1 | 89.4% | 82.4% | (many diffs) |
| 5 | human | 25924 | MYRIP | myosin VIIA and Rab interac... | NM_001284426.1 | 87% | 87.1% | (many diffs) |
| 6 | human | 25924 | MYRIP | myosin VIIA and Rab interac... | NM_001284424.2 | 81.8% | 74.9% | (many diffs) |
| 7 | human | 25924 | MYRIP | myosin VIIA and Rab interac... | NR_104316.2 | 44.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2379
- ORF length:
- 2310
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggaaggca gcaagtggaa catcctctcg aagcaccagc agtttgtgga 121 gcactgctgc atgcgctgct gctcgccctt caccttcctc gtcaacacca agcgccagtg 181 tggagattgc aaattcaatg tctgcaagag ctgctgctcc taccagaagc acgaaaaggc 241 ctgggtctgc tgcgtctgcc agcaagcgag gaggaagcct ttttgagtca aacctggaga 301 atgaaggaag catttctggc agtgattcaa cattttatag gcagtcagaa ggacatagtg 361 tgatggacac cttggctgtg gccctacggg tggctgaaga ggccattgag gaagcaattt 421 ccaaagcaga ggcatatggg gacagcctgg acaagcaaaa tgaggccagt tacctgcggg 481 accacaagga ggagctaact gaggaactgg ccacgacaat cctgcagaag attatacgaa 541 aacagaagag caaaagtagg cagcaagtgg aagaagagcc aggatggcca catccccaga 601 gttgcagcac aaaggtggca gatgagggga cctcagcatc ccctggaggc taccgtgctc 661 ccgctgccct ctggaggtcc cagtctgcct tctcaatcac tggagaagaa gccctgaaga 721 cccctccagt ggaggctcca tcgaggcagc caagggacca aggccaacac ccgagagcag 781 agtctgctct gcccagctgg aagagtgtgg acaggctgga tgaaacaaac ctggccccag 841 ttttgcagag ccccgacggg aactgggtgg ccctgaagga tggcgctcca ccccccaccc 901 gactactggc caaacctaag agcgggacgt ttcaggccct ggaggtggcc tccagtgtgg 961 catctgccta cgatgagatg ggctccgata gcgaggaaga ctttgactgg agtgaggcct 1021 tgagcaagct gtgtcccagg tcccgggccc tgcccaggaa cccccagcct cagcccacac 1081 aggcccagag ctctgaccaa ggccccatag ctgcctcccc atcctctgca ctctccccca 1141 accctgaggc catgtgctct gactcggaga cctcctccgc aggctcttcc cgagaagttg 1201 ggcaccaggc cagactgtcc tggttgcaga ggaaggcccc caggaaccct gcagctgaga 1261 agatgcgctt gcatggagag ctggacgtga acttcaaccc ccagttggcc agcagggaga 1321 cctcggacag cagcgagccg gaggaggccc cccacaccac agaccggcgg gccaggaggt 1381 ggagaagagc ccgattgggc tcagaagagc caagcaaaga accatcttcc cccagcgccc 1441 agctccggga tctagacaca catcaggtgt cggatgattt atcagagaca gacatcagca 1501 atgaggctcg ggatccccag actctcacag acaccacaga ggagaaacgg agaaacaggc 1561 tgtacgagtt agcaatgaaa atgagtgaaa aggagacttc ttcaggggag gatcaggagt 1621 ctgagcccaa gacagaatct gagaaccaga aggaaagtct gtcctctgaa gacaacagcc 1681 agagtgtcca ggaagagctg aagaagaagt tttctgctgt ttctctctgc aacatctcca 1741 cagaagtcct gaaagtcatc aatgccacag aggagttgat agcaggatct acagggccct 1801 gggagtcccc acaagtccct cctgacagac agaaggggat gtttcctcgt gggacagacc 1861 aagtgagact ggatgagcag ctgacttccc tggaagaaaa tgtatacctg gcagcaggca 1921 ctgtgtatgg actGGAGACC CAGCTGACTG AGCTAGAAGA TGCCGCCCGC TGCATCCACA 1981 GCGGCACTGA TGAGACCCAT CTGGCGGATC TGGAGGACCA GGTGGCCACG GCTGCAGCCC 2041 AAGTCCACCA TGCTGAACTC CAGATTTCAG ATATTGAGAG CCGGATTTCA GCCCTGACCA 2101 TTGCAGGATT AAACATAGCA CCATGTGTGC GCTTCACAAG AAGACGGGAT CAGAAGCAAA 2161 GGACCCAGGT ACAAACCATA GATACATCAA GGCAGCAAAG GAGGAAACTG CCTGCTCCAC 2221 CGGTGAAAGC TGAAAAAATT GAGACATCTT CAGTGACTAC CATTAAAACA TTTAACCACA 2281 ACTTCATTCT CCAAGGCTCC TCAACAAACA GGACTAAGGA AAGGAAAGGC ACCACCAAGG 2341 ATTTGATGGA GCCTGCTCTG GAGTCAGCTG TGATGTACTT GCCAACTTTC TTGTACAAAG 2401 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2461 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2521 ACGAATCACT ACACCCGGTG AGACATCCAC GCGTTAAGTC gacaatcaac ctctggatta 2581 caaaatttgt gaaagatt