Construct: ORF TRCN0000476530
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011902.1_s317c1
- Derived from:
- ccsbBroadEn_05832
- DNA Barcode:
- GCATTTTTCTCATATCGAAAGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AIRE (326)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476530
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 326 | AIRE | autoimmune regulator | NM_000383.4 | 59.3% | 49.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1113
- ORF length:
- 1044
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggttggtg tacagttccg gggcccctgg aacgcagcag cctgcaagaa 121 accgggtttt cttcccaata gggatggccc cggggggtgt ctgttggaga ccagatggat 181 ggggaacagg tggtcagggc agaatttcag gccctggcag catgggagca gggcagagac 241 tggggagttc aggtacccag agatgctgct gggggagctg ttttgggaag gaggtggctc 301 tcaggagggt gctgcacccc agcccagtct gcatgggcgt ctcttgcctg tgccagaaga 361 atgaggacga gtgtgccgtg tgtcgggacg gcggggagct catctgctgt gacggctgcc 421 ctcgggcctt ccacctggcc tgcctgtccc ctccgctccg ggagatcccc agtgggacct 481 ggaggtgctc cagctgcctg caggcaacag tccaggaggt gcagccccgg gcagaggagc 541 cccggcccca ggagccaccc gtggagaccc cgctcccccc ggggcttagg tcggcgggag 601 aggaggtaag aggtccacct ggggaacccc tagccggcat ggacacgact cttgtctaca 661 agcacctgcc ggctccgcct tctgcagccc cgctgccagg gctggactcc tcggccctgc 721 accccctact gtgtgtgggt cctgagggtc agcagaacct ggctccTGGT GCGCGTTGCG 781 GGGTGTGCGG AGATGGTACG GACGTGCTGC GGTGTACTCA CTGCGCCGCT GCCTTCCACT 841 GGCGCTGCCA CTTCCCAGCC GGCACCTCCC GGCCCGGGAC GGGCCTGCGC TGCAGATCCT 901 GCTCAGGAGA CGTGACCCCA GCCCCTGTGG AGGGGGTGCT GGCCCCCAGC CCCGCCCGCC 961 TGGCCCCTGG GCCTGCCAAG GATGACACTG CCAGTCACGA GCCCGCTCTG CACAGGGATG 1021 ACCTGGAGTC CCTTCTGAGC GAGCACACCT TCGACGGCAT CCTGCAGTGG GCCATCCAGA 1081 GCATGGCCCG TCCGGCGGCC CCCTTCCCCT CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1141 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1201 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGC 1261 ATTTTTCTCA TATCGAAAGA ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 1321 ttgtgaaaga tt