Construct: ORF TRCN0000476532
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015680.1_s317c1
- Derived from:
- ccsbBroadEn_06823
- DNA Barcode:
- GTACTTACTACTCCCACCAGCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PURB (5814)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476532
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5814 | PURB | purine rich element binding... | NM_033224.5 | 99.8% | 100% | 321T>G |
| 2 | mouse | 19291 | Purb | purine rich element binding... | NM_011221.3 | 90.3% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1005
- ORF length:
- 936
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggacggc gacagcggca gcgagcgcgg cggcggcggt gggccgtgcg 121 ggttccagcc cgcgtcccgc ggcggcggcg agcaagagac gcaggagctg gcctcgaagc 181 ggctggacat ccagaacaag cgcttctact tagatgtgaa gcagaacgcc aagggccgct 241 tcctcaagat cgccgaggtg ggcgcgggcg gttccaagag ccgcctcacg ctgtccatgg 301 cggtggccgc cgagttccgc gactcgctgg gcgacttcat agaacactac gcgcagctgg 361 gccctagcag ccccgagcag ctggcggcgg gcgccgagga gggcggcggg ccgcggcgcg 421 cgctcaagag cgaattcttg gtgcgtgaga accgcaagta ctacctggac ctcaaggaga 481 accagcgcgg ccgcttcctg cgcatccgcc aaacggtcaa ccgcggcggt ggcggcttcg 541 gcgcgggccc cgggccgggc ggcttgcaga gcggccagac catcgcgctg cctgcgcagg 601 gcctcatcga gttccgcgac gcgctggcga agctcataga cgacTACGGA GGCGAGGACG 661 ACGAGCTGGC AGGCGGCCCG GGAGGCGGCG CCGGGGGCCC AGGGGGCGGC CTGTATGGAG 721 AGCTCCCGGA GGGCACCTCC ATCACCGTGG ACTCCAAGCG CTTCTTCTTC GATGTGGGCT 781 GCAACAAATA CGGGGTGTTT CTGCGAGTGA GCGAGGTGAA GCCGTCCTAC CGCAATGCCA 841 TCACCGTACC CTTCAAAGCC TGGGGCAAGT TCGGAGGCGC CTTTTGCCGG TATGCGGATG 901 AGATGAAAGA AATCCAGGAA CGACAGAGGG ATAAGCTTTA TGAGCGACGT GGTGGGGGCA 961 GCGGCGGCGG CGAAGAGTCA GAGGGTGAGG AGGTGGATGA GGATTTGCCA ACTTTCTTGT 1021 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1081 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1141 GAAAGGACGA GTACTTACTA CTCCCACCAG CAACACGCGT TAAGTCgaca atcaacctct 1201 ggattacaaa atttgtgaaa gatt