Construct: ORF TRCN0000476545
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001164.1_s317c1
- Derived from:
- ccsbBroadEn_09076
- DNA Barcode:
- ATGAAGTAGCAATAATACTGAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACSBG2 (81616)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476545
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81616 | ACSBG2 | acyl-CoA synthetase bubbleg... | NM_001289177.2 | 99.6% | 99.2% | (many diffs) |
| 2 | human | 81616 | ACSBG2 | acyl-CoA synthetase bubbleg... | NM_001289178.2 | 99.6% | 99.2% | (many diffs) |
| 3 | human | 81616 | ACSBG2 | acyl-CoA synthetase bubbleg... | NM_030924.5 | 99.6% | 99.2% | (many diffs) |
| 4 | human | 81616 | ACSBG2 | acyl-CoA synthetase bubbleg... | XM_017027332.2 | 99.6% | 99.2% | (many diffs) |
| 5 | human | 81616 | ACSBG2 | acyl-CoA synthetase bubbleg... | XM_017027333.1 | 97% | 95.7% | (many diffs) |
| 6 | human | 81616 | ACSBG2 | acyl-CoA synthetase bubbleg... | NM_001289179.2 | 95.5% | 95.3% | (many diffs) |
| 7 | human | 81616 | ACSBG2 | acyl-CoA synthetase bubbleg... | NM_001321384.2 | 92.1% | 91.7% | (many diffs) |
| 8 | human | 81616 | ACSBG2 | acyl-CoA synthetase bubbleg... | XM_017027334.1 | 91.2% | 90.8% | (many diffs) |
| 9 | human | 81616 | ACSBG2 | acyl-CoA synthetase bubbleg... | XM_005259653.3 | 84.8% | 83.9% | (many diffs) |
| 10 | human | 81616 | ACSBG2 | acyl-CoA synthetase bubbleg... | NM_001289180.1 | 71.6% | 71.3% | (many diffs) |
| 11 | human | 81616 | ACSBG2 | acyl-CoA synthetase bubbleg... | XM_011528329.1 | 66.3% | 63.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2064
- ORF length:
- 1998
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tggaacccca aagactcaag aaggagctaa agatcttgaa gtagacatga 121 ataaaacaga agttactccc aggctgtgga ccacctgtcg agatggagaa gtccttctga 181 ggctatccaa acacggacca ggccatgaga ccccgatgac catccctgaa ttttttcgag 241 agtcagtcaa ccgatttgga acttatccag ccctcgcatc caagaatggc aaaaagtggg 301 aaattctgaa tttcaaccag tactatgagg cttgtcggaa ggctgcaaaa tccttgatca 361 agctgggttt ggagcgtttc cacggagttg gtatcctggg gtttaactct gcagagtggt 421 ttatcactgc tgttggtgcc atcctagccg ggggtctttg tgttggtatt tatgccacca 481 actctgccga ggcttgtcaa tatgtcatca ctcatgccaa agtgaacatc ttgctggttg 541 agaatgatca acagttacag aaaatccttt cgattccaca gagcagccta gagcccctaa 601 aagcgatcat ccagtacaga ctgccaatga agaagaacaa caacttgtac tcttgggatg 661 atttcatgga acttggcaga agtatccctg acacccaact ggagcaggtc atcgagagcc 721 agaaggcgaa tcaatgcgca gtgctcatct acacttcagg gaccacaggc atacccaagg 781 gagtgatgct cagtcatgac aacatcacgt ggattgcagg agcagtgaca aaggacttta 841 aactgacaga caagcatgag acggtggtta gctacctccc actcagccat attgcagcac 901 agatgatgga catctgggta cccataaaga ttggggcgct cacatacttt gctcaagcag 961 atgctctcaa gggcaccttg gtaagtactc taaaggaggt aaaacctact gtcttcattg 1021 gagtgcctca aatttgggag aagatacatg agatggtgaa gaaaaatagt gccaagtcca 1081 tgggcttgaa gaagaaggca ttcgtgtggg caagaaacat tggcttcaag gtcaactcaa 1141 aaaagatgtt ggggaaatat aatactcccg tgagctaccg catggctaag actctcgtgt 1201 tcagcaaagt caagacatcc cttggcttgg atcactgtca ctcttttatc agtgggactg 1261 cgcccctcaa ccaagagact gccgagttct ttctaagctt ggacatacct ataggcgagt 1321 tgtatgggtt gagtgaaagc tcgggacccc acacgatatc caaccagaat aactacaggc 1381 ttctaagctg tggcaagatc ttgactgggt gtaagaatat gctgttccag cagaacaagg 1441 atggcattgg ggagatctgc ctctggggta ggcacatctt catgggctat ctggaaagtg 1501 agactgaaac tacagaggcc atcgatgatg aaggctggct acactctggg gatctgggcc 1561 agctggacgg tctgggtttc ctctatgtca ccggccacat caaagaaatc cttatcactg 1621 ctggtggtga aaatgtgccc cccattcctg ttgagacctt ggttaagaag aagaTCCCCA 1681 TCATCAGTAA CGCCATGTTA GTAGGAGATA AACTGAAGTT TCTGAGCATG TTGCTGACGC 1741 TGAAGTGTGA GATGAATCAG ATGAGCGGAG AACCTCTGGA CAAGCTGAAC TTTGAGGCCA 1801 TCAACTTCTG TCGGGATCTG GACAGCCAGG CATCCACCGT GACTGAGATT GTGAAGCAGC 1861 AAGACCCCCT GGTCTACAAG GCCATCCAGC AAGGCATCAA TGCTGTGAAC CAGGAAGCCA 1921 TGAACAATGC ACAGAAGATT CAAAAGTGGG TCATCTTGGA GAAGGACTTT TCCATCTATG 1981 GTGGAGAGCT AGGTCCAATG ATGAAACTTA AGAGACATTT TGTAGCCCAG AAATACAAAA 2041 AACAAATTGA TCACATGTAC CACTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2101 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2161 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TGAAGTAGCA 2221 ATAATACTGA AATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2281 att