Construct: ORF TRCN0000476564
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017635.1_s317c1
- Derived from:
- ccsbBroadEn_00059
- DNA Barcode:
- CAATGCGGGAATGAAGTTATCGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALX3 (257)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476564
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 257 | ALX3 | ALX homeobox 3 | NM_006492.3 | 100% | 100% | |
| 2 | mouse | 11694 | Alx3 | aristaless-like homeobox 3 | NM_007441.3 | 88.8% | 91.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1098
- ORF length:
- 1029
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaccccgag cactgcgcgc ctttccgcgt ggggcctgca cccggcccct 121 atgtggcctc gggggacgag cctccgggcc cgcagggaac ccccgccgct gcgcctcacc 181 tgcaccccgc gccgccccgc ggcccgcggc tgacccgctt tccggcctgc gggcccctgg 241 agccctacct cccagagccg gccaagccgc ccgccaagta cctgcaggac ctcgggcccg 301 gcccggccct caacggcggc cacttctacg agggccccgc ggaagctgag gagaagacct 361 ccaaagctgc cagcttcccc cagctgccct tggactgccg agggggcccc agagacgggc 421 cctctaactt gcaaggctcc ccaggcccct gcctggccag cctgcatctt cctctttccc 481 cgggactccc tgactccatg gagttggcca agaacaagag caagaagcgt cgtaaccgca 541 cgaccttcag cacattccag ctggaggagc tggagaaggt cttccagaaa acccactatc 601 ctgatgtgta tgcccgggag cagctggccc tgcgcacaga cctgactgag gcccgggtac 661 aggtctggtt ccagaaccgc agagccaagt ggcggaagcg cgagcgttat gggaagatcc 721 aggaggggcg gaaccccttc acggctgcct atgacatctc tgtgctgccc cgtactgaca 781 gccacccTCA GCTGCAGAAC TCCCTGTGGG CCAGTCCAGG ATCTGGGAGC CCTGGAGGCC 841 CCTGCCTTGT GTCTCCAGAG GGCATCCCCT CCCCATGCAT GTCTCCATAT TCCCACCCCC 901 ATGGGAGTGT GGCTGGCTTC ATGGGGGTGC CAGCCCCTTC TGCGGCTCAC CCTGGCATCT 961 ACTCCATCCA TGGCTTTCCC CCCACCCTGG GGGGCCACAG CTTTGAGCCT TCCTCAGATG 1021 GTGACTATAA GTCTCCAAGC CTCGTCTCGC TCAGGGTAAA GCCCAAGGAG CCACCCGGCC 1081 TTCTGAACTG GACCACGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1141 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGCCCG TAACTTGAAA 1201 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACAATGCG GGAATGAAGT 1261 TATCGGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt