Construct: ORF TRCN0000476587
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002982.1_s317c1
- Derived from:
- ccsbBroadEn_01818
- DNA Barcode:
- CTCTGCAATCATCATATCGTTACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF200 (7752)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476587
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7752 | ZNF200 | zinc finger protein 200 | NM_003454.4 | 100% | 100% | |
| 2 | human | 7752 | ZNF200 | zinc finger protein 200 | NM_198088.3 | 100% | 100% | |
| 3 | human | 7752 | ZNF200 | zinc finger protein 200 | XM_005255556.1 | 100% | 100% | |
| 4 | human | 7752 | ZNF200 | zinc finger protein 200 | XM_006720941.2 | 100% | 100% | |
| 5 | human | 7752 | ZNF200 | zinc finger protein 200 | NM_001145446.2 | 99.7% | 99.7% | 465_466insGCA |
| 6 | human | 7752 | ZNF200 | zinc finger protein 200 | NM_001145447.2 | 99.7% | 99.7% | 465_466insGCA |
| 7 | human | 7752 | ZNF200 | zinc finger protein 200 | NM_001145448.2 | 99.7% | 99.7% | 465_466insGCA |
| 8 | human | 7752 | ZNF200 | zinc finger protein 200 | NM_198087.3 | 99.7% | 99.7% | 336_337insGAG |
| 9 | human | 7752 | ZNF200 | zinc finger protein 200 | XM_017023648.1 | 41.3% | 39.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1251
- ORF length:
- 1185
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ggctgcaaaa gtggttccta tgcccccaaa gccaaagcag tcctttatac 121 tgagagttcc gccagactcc aagctgggcc aagacctact tcgagatgcc actaacgggc 181 ccaagaccat ccaccagcta gtgctggagc acttcctcac cttcttgccc aagccaagcc 241 tggtccagcc cagtcagaaa gtcaaggaga ccttggttat tatgaaagat gtgagctcaa 301 gccttcagaa cagagtgcat cctcgtccct tggtgaagct tctgcccaaa ggagtccaaa 361 aggaacaaga gacagtgtct ctgtatttga aagctaaccc tgaggagctg gtggtctttg 421 aggatttgaa tgtatttcac tgccaggaag aatgtgtgag cttggatcct actcaacaac 481 tcacgtcaga gaaggaagat gacagcagtg tcggggaaat gatgttactg gcagtcaatg 541 gcagtaatcc tgaaggtgaa gatcctgaga gggaacctgt agaaaatgaa gattatagag 601 aaaagtcttc agatgatgat gaaatggatt cttccttggt ctctcagcag cctcccgata 661 accaggaaaa ggaacgacta aatacatcca ttccacaaaa aaggaaaatg agaaatctgt 721 tagttaccat tgagaatgat actcctctag aggaactctc aaaatatgta gacatcagta 781 ttattgccct tactcgaaat cggaggacaa ggagatggta cacttgtcca ctgtgtggga 841 aacagtttaa tgaaagttct tacctcattt cccaccagag gaccCACACT GGAGAAAAAC 901 CCTATGACTG TAATCACTGT GGGAAAAGCT TCAATCATAA AACAAACCTC AATAAACATG 961 AGCGAATTCA TACAGGAGAG AAACCTTATT CCTGTTCTCA GTGTGGAAAA AACTTCCGTC 1021 AGAATTCTCA TCGGAGTCGT CATGAAGGAA TCCATATAAG GGAGAAGATA TTTAAGTGTC 1081 CAGAATGTGG GAAAACCTTC CCAAAGAATG AGGAGTTTGT GCTTCATCTG CAGAGTCATG 1141 AGGCTGAGAG ACCATATGGT TGCAAAAAAT GTGGGAGAAG ATTTGGTCGG CTGTCAAACT 1201 GTACCCGGCA TGAGAAAACC CACTCAGCCT GTAAGACCCG AAAGCAGAAG TACCCAACTT 1261 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1321 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1381 CTTGTGGAAA GGACGACTCT GCAATCATCA TATCGTTACC ACGCGTTAAG TCgacaatca 1441 acctctggat tacaaaattt gtgaaagatt