Construct: ORF TRCN0000476617
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012975.1_s317c1
- Derived from:
- ccsbBroadEn_02228
- DNA Barcode:
- CTGTATGCATCGAATTCGATGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EMC2 (9694)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476617
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9694 | EMC2 | ER membrane protein complex... | NM_014673.5 | 100% | 100% | |
| 2 | human | 9694 | EMC2 | ER membrane protein complex... | NM_001329493.1 | 97% | 97% | 155_181del |
| 3 | human | 9694 | EMC2 | ER membrane protein complex... | NM_001329495.1 | 97% | 95.9% | (many diffs) |
| 4 | human | 9694 | EMC2 | ER membrane protein complex... | NM_001329494.1 | 66.1% | 59.3% | (many diffs) |
| 5 | human | 9694 | EMC2 | ER membrane protein complex... | NR_138033.1 | 23.3% | 1_76del;381_382ins58;910_3502del | |
| 6 | mouse | 66736 | Emc2 | ER membrane protein complex... | NM_025736.2 | 92% | 97.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 957
- ORF length:
- 891
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaaggtctca gagctttacg atgtcacttg ggaagaaatg agagataaaa 121 tgagaaaatg gagagaagaa aactcaagaa atagtgagca aattgtggaa gttggagaag 181 aattaattaa tgaatatgct tctaagctgg gagatgatat ttggatcata tatgaacagg 241 tgatgattgc agcactagac tatggtcggg atgacttggc attgttttgt cttcaagagc 301 tgagaagaca gttccctggc agtcacagag tcaagcgatt aacaggcatg agatttgaag 361 ccatggaaag atatgatgat gctatacagc tatatgatag gattttacaa gaagatccaa 421 ctaacactgc tgcaagaaag cgtaagattg ccattcgaaa agcccagggg aaaaatgtgg 481 aggccattcg ggagctgaat gagtatctgg aacaatttgt tggagaccaa gaagccTGGC 541 ATGAACTTGC AGAACTTTAC ATCAATGAAC ATGACTATGC AAAAGCAGCC TTTTGTTTAG 601 AGGAACTAAT GATGACTAAT CCACACAACC ACTTATACTG TCAGCAGTAT GCTGAAGTTA 661 AGTATACCCA AGGTGGACTT GAAAACCTCG AACTTTCAAG AAAGTATTTT GCACAGGCAT 721 TGAAACTGAA CAACAGAAAT ATGAGAGCTT TGTTTGGACT TTATATGTCG GCAAGTCATA 781 TTGCTTCTAA TCCAAAAGCA AGTGCAAAAA CGAAAAAGGA CAACATGAAA TATGCTAGTT 841 GGGCAGCTAG TCAAATAAAC AGAGCTTATC AGTTTGCAGG TCGAAGTAAG AAGGAAACCA 901 AATATTCTCT TAAGGCTGTC GAAGACATGT TGGAAACATT GCAGATCACC CAGTCTTACC 961 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1021 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1081 ATATATCTTG TGGAAAGGAC GACTGTATGC ATCGAATTCG ATGCCTACGC GTTAAGTCga 1141 caatcaacct ctggattaca aaatttgtga aagatt