Construct: ORF TRCN0000476646
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012979.1_s317c1
- Derived from:
- ccsbBroadEn_15303
- DNA Barcode:
- TAAGCATACTCGGTGAAAACATAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TAS2R60 (338398)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476646
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 338398 | TAS2R60 | taste 2 receptor member 60 | NM_177437.1 | 99.3% | 47.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 618
- ORF length:
- 549
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaatggagac cacatggttc taggatcttc ggtgactgac aagaaggcca 121 tcatcttggt taccatttta ctccttttac gcctggtagc aatagcaggc aatggcttca 181 tcactgctgc tctgggcgtg gagtgggtgc tacggagaat gttgttgcct tgtgataagt 241 tattggttag cctaggggcc tctcgcttct gtctgcagtc agtggtaatg ggtaagacca 301 tttatgtttt cttgcatccg atggccttcc catacaaccc tgtactgcag tttctagctt 361 tccagtggga cttcctgaat gctgccacct tatggtcctc tacctggctc agtgtcttct 421 attgtgtgaa aattgctacc ttcacccacc ctgtcttctt ctggctaaag cacaagttgt 481 ctgggtggct accatggatg ctcttcagca actgcaggct tctccagctt caccaccatt 541 ctatttttca taggcaacca cagaatgtat cagaactatt taaggaacca tctacaacct 601 tggaatgtca ctggcgatag catacggagc tactgtgaga aattctatct cttccctcta 661 aaaatgatta cttggacaat gcccactgct gtctttttca tttgcatgat tttgctcatc 721 acatctctgg gaagacacag gaagaaggct ctccttacaa cctcaggatt ccgagagccc 781 agtgtgcagg cacacataaa ggctctgctg gctctcctct cttttgccat gctcttcatc 841 tcatatttcc tgtcactggt gttcagtgct gcaggtattt ttccacctct ggactttaaa 901 ttctgggtgt gggagtcagt gatttATCTG TGTGCAGCAG TTCACCCCAT CATTCTGCTC 961 TTCAGCAACT GCAGGCTGAG AGCTGTGCTG AAGAGTCGCC GTTCCTCAAG GTGTGGGACA 1021 CCTTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC 1081 CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT 1141 TGGCTTTATA TATCTTGTGG AAAGGACGAT AAGCATACTC GGTGAAAACA TAGACGCGTT 1201 AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att