Construct: ORF TRCN0000476665
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002120.1_s317c1
- Derived from:
- ccsbBroadEn_04753
- DNA Barcode:
- CACTTGATCGCAACAATCCCTCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERP27 (121506)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476665
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 121506 | ERP27 | endoplasmic reticulum prote... | NM_152321.4 | 100% | 100% | |
| 2 | human | 121506 | ERP27 | endoplasmic reticulum prote... | NM_001300784.1 | 61.8% | 60% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 885
- ORF length:
- 819
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agctgccccg tccaggttca tgttcctctt atttctcctc acgtgtgagc 121 tggctgcaga agttgctgca gaagttgaga aatcctcaga tggtcctggt gctgcccagg 181 aacccacgtg gctcacagat gtcccagctg ccatggaatt cattgctgcc actgaggtgg 241 ctgtcatagg cttcttccag gatttagaaa taccagcagt gcccatactc catagcatgg 301 tgcaaaaatt cccaggcgtg tcatttggga tcagcactga ttctgaggtt ctgacacact 361 acaacatcac tgggaacacc atctgcctct ttcgcctggt agacaatgaa caactgaatt 421 tagaggacga agacattgaa agcattgatg ccaccaaatt gagccgtttC ATTGAGATCA 481 ACAGCCTCCA CATGGTGACA GAGTACAACC CTGTGACTGT GATTGGGTTA TTCAACAGCG 541 TAATTCAGAT TCATCTCCTC CTGATAATGA ACAAGGCCTC CCCAGAGTAT GAAGAGAACA 601 TGCACAGATA CCAGAAGGCA GCCAAGCTCT TCCAGGGGAA GATTCTCTTT ATTCTGGTGG 661 ACAGTGGTAT GAAAGAAAAT GGGAAGGTGA TATCATTTTT CAAACTAAAG GAGTCTCAAC 721 TGCCAGCTTT GGCAATTTAC CAGACTCTAG ATGACGAGTG GGATACACTG CCCACAGCAG 781 AAGTTTCCGT AGAGCATGTG CAAAACTTTT GTGATGGATT CCTAAGTGGA AAATTGTTGA 841 AAGAAAATCG TGAATCAGAA GGAAAGACTC CAAAGGTGGA ACTCTGCCCA ACTTTCTTGT 901 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 961 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1021 GAAAGGACGA CACTTGATCG CAACAATCCC TCTAACGCGT TAAGTCgaca atcaacctct 1081 ggattacaaa atttgtgaaa gatt