Construct: ORF TRCN0000476671
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004127.1_s317c1
- Derived from:
- ccsbBroadEn_12451
- DNA Barcode:
- TTTCGACACCTCCACATTCTTAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF9 (64147)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476671
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64147 | KIF9 | kinesin family member 9 | XM_017007031.1 | 13.7% | 13.3% | (many diffs) |
| 2 | human | 64147 | KIF9 | kinesin family member 9 | XM_011534005.2 | 12.4% | 12.1% | (many diffs) |
| 3 | human | 64147 | KIF9 | kinesin family member 9 | XM_017007030.1 | 12.4% | 12.1% | (many diffs) |
| 4 | human | 64147 | KIF9 | kinesin family member 9 | NM_022342.5 | 12.3% | 12% | (many diffs) |
| 5 | human | 64147 | KIF9 | kinesin family member 9 | XM_006713291.3 | 11.9% | 11.6% | (many diffs) |
| 6 | human | 64147 | KIF9 | kinesin family member 9 | XM_005265389.4 | 11.7% | 11.4% | (many diffs) |
| 7 | human | 64147 | KIF9 | kinesin family member 9 | NM_001134878.2 | 11.3% | 11% | (many diffs) |
| 8 | human | 64147 | KIF9 | kinesin family member 9 | NM_182902.3 | 11.3% | 11% | (many diffs) |
| 9 | human | 64147 | KIF9 | kinesin family member 9 | XM_011534004.2 | 11.3% | 11% | (many diffs) |
| 10 | human | 64147 | KIF9 | kinesin family member 9 | XM_017007029.1 | 11.3% | 11% | (many diffs) |
| 11 | human | 64147 | KIF9 | kinesin family member 9 | XM_011534003.2 | 11.1% | 10.8% | (many diffs) |
| 12 | human | 64147 | KIF9 | kinesin family member 9 | XM_005265388.4 | 10.8% | 10.5% | (many diffs) |
| 13 | human | 64147 | KIF9 | kinesin family member 9 | XR_002959567.1 | 10.3% | (many diffs) | |
| 14 | human | 64147 | KIF9 | kinesin family member 9 | XR_940489.2 | 9.8% | (many diffs) | |
| 15 | human | 64147 | KIF9 | kinesin family member 9 | XR_940488.2 | 9.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 363
- ORF length:
- 273
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGGTACTAG GAAAAAAGTT CATGCATTTG 121 TCCGTGTCAA ACCCACCGAT GACTTTGCTC ATGAAATGAT CAGATACGGA GATGACAAAA 181 GAAGCATTGA TATTCACTTA AAAAAAGACA TTCGGAGAGG AGTTGTCAAT AACCAACAGA 241 CAGACTGGTC GTTTAAGTTG GATGGAGTTC TTCACGATGC CTCCCAGGAC TTGGTTTATG 301 AGACAGTTGC AAAGGATGTG GTTTCTCAGG CCCTCGATGG CTATAATGGT AACTTAATTA 361 ATTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 421 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 481 GGCTTTATAT ATCTTGTGGA AAGGACGATT TCGACACCTC CACATTCTTA GTACGCGTTA 541 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt