Construct: ORF TRCN0000476675
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001300.1_s317c1
- Derived from:
- ccsbBroadEn_07748
- DNA Barcode:
- AACGGAAAATCCTTTTAAACAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADAMTS5 (11096)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476675
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11096 | ADAMTS5 | ADAM metallopeptidase with ... | NM_007038.5 | 99.8% | 99.5% | (many diffs) |
| 2 | human | 11096 | ADAMTS5 | ADAM metallopeptidase with ... | XM_024452053.1 | 99.8% | 99.5% | (many diffs) |
| 3 | human | 11096 | ADAMTS5 | ADAM metallopeptidase with ... | XM_024452054.1 | 93.8% | 93.5% | (many diffs) |
| 4 | mouse | 23794 | Adamts5 | a disintegrin-like and meta... | NM_011782.2 | 85% | 89.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2859
- ORF length:
- 2790
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgctcggg tgggcgtccc tgctgctgtg cgcgttccgc ctgcccctgg 121 ccgcggtcgg ccccgccgcg acacctgccc aggataaagc cgggcagcct ccgactgctg 181 cagcagccgc ccagccccgc cggcggcagg gggaggaggt gcaggagcga gccgagcctc 241 ccggccaccc gcaccccctg gcgcagcggc gcaggagcaa ggggctggtg cagaacatcg 301 accaactcta ctccggcggc ggcaaggtgg gctacctcgt ctacgcgggc ggccggaggt 361 tcctcttgga cctggagcga gatggttcgg tgggcattgc tggcttcgtg cccgcaggag 421 gcgggacgag tgcgccctgg cgccaccgga gccactgctt ctatcggggc acagtggacg 481 ctagtccccg ctctctggct gtctttgacc tctgtggggg tctcgacggc ttcttcgcgg 541 tcaagcacgc gcgctacacc ctaaagccac tgctgcgcgg accctgggcg gaggaagaaa 601 aggggcgcgt gtacggggat gggtccgcac ggatcctgca cgtctacacc cgcgagggct 661 tcagcttcga ggccctgccg ccgcgcgcca gctgcgaaac ccccgcgtcc acaccggagg 721 cccacgagca tgctccggcg cacagcaacc cgagcggacg cgcagcactg gcctcgcagc 781 tcttggacca gtccgctctc tcgcccgctg ggggctcagg accgcagacg tggtggcggc 841 ggcggcgccg ctccatctcc ctggcccgcc aggtggagct gcttctggtg gctgacgcgt 901 ccatggcgcg gttgtatggc cggggcctgc agcattacct gctgaccctg gcctccatcg 961 ccaataggct gtacagccat gctagcatcg agaaccacat ccgcctggcc gtggtgaagg 1021 tggtggtgct aggcgacaag gacaagagcc tggaagtgag caagaacgct gccaccacac 1081 tcaagaactt ttgcaagtgg cagcaccaac acaaccagct gggagatgac catgaggagc 1141 actacgatgc agctatcctg tttactcggg aggatttatg tgggcatcat tcatgtgaca 1201 ccctgggaat ggcagacgtt gggaccatat gttctccaga gcgcagctgt gctgtgattg 1261 aagacgatgg cctccacgca gccttcactg tggctcacga aatcggacat ttacttggcc 1321 tctcccatga cgattccaaa ttctgtgaag agacctttgg ttccacagaa gataagcgct 1381 taatgtcttc catccttacc agcattgatg catctaagcc ctggtccaaa tgcacttcag 1441 ccaccatcac agaattcctg gatgatggcc atggtaactg tttgctggac ctaccacgaa 1501 agcagatcct gggccccgaa gaactcccag gacagaccta cgatgccacc cagcagtgca 1561 acctgacatt cgggcctgag tactccgtgt gtcccggcat ggatgtctgt gctcgcctgt 1621 ggtgtgctgt ggtacgccag ggccagatgg tctgtctgac caagaagctg cctgcggtgg 1681 aagggacgcc ttgtggaaag gggagaatct gcctgcaggg caaatgtgtg gacaaaacca 1741 agaaaaaata ttattcaacg tcaagccatg gcaactgggg atcttgggga tcctggggcc 1801 agtgttctcg ctcatgtgga ggaggagtgc agtttgccta tcgtcactgt aataaccctg 1861 ctcccagaaa caacggacgc tactgcacag ggaagagggc catctaccgc tcctgcagtc 1921 tcatgccctg cccacccaat ggtaaatcat ttcgtcatga acagtgtgag gccaaaaatg 1981 gctatcagtc tgatgcaaaa ggagtcaaaa cttttgtgga atgggttccc aaatatgcag 2041 gtgtcctgcc agcggatgtg tgcaagctga cctgcagagc caagggcact ggctactatg 2101 tggtattttc tccaaaggtg accgatggca ctgaatgtag gccgtacagt aattccgtct 2161 gcgtccgggg gaagtgtgtg agaactggct gtgacggcat cattggctca aagctgcagt 2221 atgacaagtg cggagtatgt ggaggagaca actccagctg tacaaagatt gttggaacct 2281 ttaataagaa aagtaagggt tacactgacg tggtgaggat tcctgaaggg gcaacccaca 2341 taaaagttcg acagttcaaa gccaaagacc agactagatt cactgcctat ttagccctga 2401 aaaagaaaaa cggtgagtac cttatcaatg gaaagtacat gatctccact tcagagacta 2461 tcattgacat caatggaaca gtcatgaact atagcggttG GAGCCACAGG GATGACTTCC 2521 TGCATGGCAT GGGCTACTCT GCCACGAAGG AAATTCTAAT AGTGCAGATT CTTGCAACAG 2581 ACCCCACTAA ACCATTAGAT GTCCGTTATA GCTTTTTTGT TCCCAAGAAG TCCACTCCAA 2641 AAGTAAACTC TGTCACTAGT CATGGCAGCA ATAAAGTGGG ATCACACACT TCGCAGCCGC 2701 AGTGGGTCAC GGGCCCATGG CTCGCCTGCT CTAGGACCTG TGACACAGGT TGGCACACCA 2761 GAACGGTGCA GTGCCAGGAT GGAAACCGGA AGTTAGCAAA AGGATGTCCT ATCTCCCAAA 2821 GGCCTTCTGC GTTTAAGCAA TGCTTGTTGA AGAAATGTTT GCCAACTTTC TTGTACAAAG 2881 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2941 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 3001 ACGAAACGGA AAATCCTTTT AAACAAAAAC GCGTTAAGTC gacaatcaac ctctggatta 3061 caaaatttgt gaaagatt