Construct: ORF TRCN0000476680
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010566.1_s317c1
- Derived from:
- ccsbBroadEn_07913
- DNA Barcode:
- TGCATTAGAGCTAATCTACGCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PATZ1 (23598)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476680
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23598 | PATZ1 | POZ/BTB and AT hook contain... | NM_032051.2 | 99.9% | 100% | 129G>A |
| 2 | human | 23598 | PATZ1 | POZ/BTB and AT hook contain... | NM_032052.2 | 87.5% | 81.4% | (many diffs) |
| 3 | human | 23598 | PATZ1 | POZ/BTB and AT hook contain... | NM_032050.2 | 77.9% | 68.6% | (many diffs) |
| 4 | human | 23598 | PATZ1 | POZ/BTB and AT hook contain... | NM_014323.3 | 72.9% | 65.4% | (many diffs) |
| 5 | mouse | 56218 | Patz1 | POZ (BTB) and AT hook conta... | NM_001253691.1 | 83% | 89% | (many diffs) |
| 6 | mouse | 56218 | Patz1 | POZ (BTB) and AT hook conta... | NM_001253690.1 | 78.6% | 80.7% | (many diffs) |
| 7 | mouse | 56218 | Patz1 | POZ (BTB) and AT hook conta... | NM_019574.3 | 71.8% | 67.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1677
- ORF length:
- 1611
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcgggtgaac gacgcttcgt gcggcccgtc tggctgctac acataccagg 121 tgagcagaca cagcacggag atgctgcaca acctgaacca gcagcgcaaa aacggcgggc 181 gcttctgcga cgtactcttg cgggtaggcg acgagagctt cccagcgcac cgcgccgtgc 241 tggccgcctg cagcgagtac tttgagtcgg tgttcagcgc ccagttgggc gacggcggag 301 ctgcggacgg gggtccggct gatgtagggg gcgcgacggc agcaccaggc ggcggggccg 361 ggggcagccg ggagctggag atgcacacta tcagctccaa ggtatttggg gacattctgg 421 acttcgccta cacttcccgc atcgtggtgc gcttggagag ctttcccgaa ctcatgacgg 481 ccgccaagtt cctgctgatg aggtcggtta tcgagatctg ccaggaagtc atcaaacagt 541 ccaacgtaca gatcctggta ccccctgccc gcgccgatat aatgctcttt cgcccccctg 601 ggacctcgga cttgggcttc cctttggaca tgaccaacgg ggcagccttg gcagccaaca 661 gcaatggcat cgccggcagc atgcagccag aggaggaggc agctcgggcg gctggtgcag 721 ccattgcagg ccaagcctct ttgcctgtgt tacctggggt ggaccgcttg cccatggtgg 781 ctggacccct atccccccaa ctgctgactt ccccattccc cagtgtggca tccagtgccc 841 ctcccctgac tggcaagcga ggccggggcc gcccaaggaa ggccaacctg ctggactcaa 901 tgtttgggtc cccagggggc ctgagggagg caggcatcct tccatgcggt ctatgtggta 961 aggtgttcac tgatgccaac cggctccggc agcacgaggc ccagcacggt gtcaccagcc 1021 tccagctggg ctacatcgac cttcctcctc cgaggctggg tgagaatggg ctacccatct 1081 ctgaagaccc cgacggcccc cgaaagagga gccggaccag gaagcaggtg gcttgtgaga 1141 tctgcggcaa gatcttccgt gatgtgtatc atcttaaccg gcacaagctg tcccactctg 1201 gggagaagcc ctactcctgc cctgtgtgtg ggttgcggtt caagagaaaa gaccgcatgt 1261 cctaccatgt gcggtcccat gatgggtccg tgggcaagcc ttacatctgc cagagctgtg 1321 ggaaaggctt ctccaggcct gatcacttga acggacatat caagcaggtg cacacttcTG 1381 AGCGGCCTCA CAAGTGTCAG GTGTGGGTTG GGAGCAGCAG CGGCCTGCCG CCCCTGGAAC 1441 CTCTTCCTAG CGACCTGCCA TCATGGGACT TTGCCCAGCC TGCTTTGTGG AGGTCGTCCC 1501 ATTCGGTTCC TGACACCGCC TTTTCCCTTT CTCTAAAAAA ATCATTCCCA GCCCTTGAAA 1561 ACCTGGGCCC AGCACACTCC AGCAACACTC TCTTCTGCCC AGCCCCGCCG GGATATCTGA 1621 GGCAGGGCTG GACCACCCCA GAGGGCAGCA GGGCCTTTAC CCAGTGGCCT GTTGGCTACC 1681 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1741 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1801 ATATATCTTG TGGAAAGGAC GATGCATTAG AGCTAATCTA CGCGCGACGC GTTAAGTCga 1861 caatcaacct ctggattaca aaatttgtga aagatt