Construct: ORF TRCN0000476681
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009381.1_s317c1
- Derived from:
- ccsbBroadEn_06401
- DNA Barcode:
- ACAGTAACAACTGCGATTCACGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HRH1 (3269)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476681
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3269 | HRH1 | histamine receptor H1 | NM_000861.3 | 99.9% | 99.7% | 923T>A |
| 2 | human | 3269 | HRH1 | histamine receptor H1 | NM_001098211.2 | 99.9% | 99.7% | 923T>A |
| 3 | human | 3269 | HRH1 | histamine receptor H1 | NM_001098212.2 | 99.9% | 99.7% | 923T>A |
| 4 | human | 3269 | HRH1 | histamine receptor H1 | NM_001098213.2 | 99.9% | 99.7% | 923T>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1527
- ORF length:
- 1461
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cctccccaat tcctcctgcc tcttagaaga caagatgtgt gagggcaaca 121 agaccactat ggccagcccc cagctgatgc ccctggtggt ggtcctgagc actatctgct 181 tggtcacagt agggctcaac ctgctggtgc tgtatgccgt acggagtgag cggaagctcc 241 acactgtggg gaacctgtac atcgtcagcc tctcggtggc ggacttgatc gtgggtgccg 301 tcgtcatgcc tatgaacatc ctctacctgc tcatgtccaa gtggtcactg ggccgtcctc 361 tctgcctctt ttggctttcc atggactatg tggccagcac agcgtccatt ttcagtgtct 421 tcatcctgtg cattgatcgc taccgctctg tccagcagcc cctcaggtac cttaagtatc 481 gtaccaagac ccgagcctcg gccaccattc tgggggcctg gtttctctct tttctgtggg 541 ttattcccat tctaggctgg aatcacttca tgcagcagac ctcggtgcgc cgagaggaca 601 agtgtgagac agacttctat gatgtcacct ggttcaaggt catgactgcc atcatcaact 661 tctacctgcc caccttgctc atgctctggt tctatgccaa gatctacaag gccgtacgac 721 aacactgcca gcaccgggag ctcatcaata ggtccctccc ttccttctca gaaattaagc 781 tgaggccaga gaaccccaag ggggatgcca agaaaccagg gaaggagtct ccctgggagg 841 ttctgaaaag gaagccaaaa gatgctggtg gtggatctgt cttgaagtca ccatcccaaa 901 cccccaagga gatgaaatcc ccagttgtct tcagccaaga ggatgataga gaagtagaca 961 aactctactg ctttccactt gatattgagc acatgcaggc tgcggcagag gggagtagca 1021 gggactatgt agccgtcaac cggagccatg gccagctcaa gacagatgag cagggcctga 1081 acacacatgg ggccagcgag atatcagagg atcagatgtt aggtgatagc caatccttct 1141 ctcgaacgga ctcagaTACC ACCACAGAGA CAGCACCAGG CAAAGGCAAA TTGAGGAGTG 1201 GGTCTAACAC AGGCCTGGAT TACATCAAGT TTACTTGGAA GAGGCTCCGC TCGCATTCAA 1261 GACAGTATGT ATCTGGGTTG CACATGAACC GCGAAAGGAA GGCCGCCAAA CAGTTGGGTT 1321 TTATCATGGC AGCCTTCATC CTCTGCTGGA TCCCTTATTT CATCTTCTTC ATGGTCATTG 1381 CCTTCTGCAA GAACTGTTGC AATGAACATT TGCACATGTT CACCATCTGG CTGGGCTACA 1441 TCAACTCCAC ACTGAACCCC CTCATCTACC CCTTGTGCAA TGAGAACTTC AAGAAGACAT 1501 TCAAGAGAAT TCTGCATATT CGCTCCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1561 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1621 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACAGTAAC 1681 AACTGCGATT CACGCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1741 aagatt