Transcript: Human NM_001098212.2

Homo sapiens histamine receptor H1 (HRH1), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
HRH1 (3269)
Length:
4617
CDS:
98..1561

Additional Resources:

NCBI RefSeq record:
NM_001098212.2
NBCI Gene record:
HRH1 (3269)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001098212.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000009062 CGCATTCAAGACAGTATGTAT pLKO.1 1284 CDS 100% 5.625 7.875 N HRH1 n/a
2 TRCN0000009065 CGTCGTCATGCCTATGAACAT pLKO.1 331 CDS 100% 4.950 6.930 N HRH1 n/a
3 TRCN0000271228 AGTGGCTAAGTGTCCATTATT pLKO_005 3721 3UTR 100% 15.000 12.000 N HRH1 n/a
4 TRCN0000271226 GCACCAGGCAAAGGCAAATTG pLKO_005 1205 CDS 100% 13.200 10.560 N HRH1 n/a
5 TRCN0000284355 AGCACCGGGAGCTCATCAATA pLKO_005 762 CDS 100% 13.200 9.240 N HRH1 n/a
6 TRCN0000011675 CCCTGGAAATTCTGCCTTATT pLKO.1 2179 3UTR 100% 13.200 9.240 N HRH1 n/a
7 TRCN0000009064 CCTCCCTTCCTTCTCAGAAAT pLKO.1 787 CDS 100% 13.200 9.240 N HRH1 n/a
8 TRCN0000271227 TACTGCTTTCCACTTGATATT pLKO_005 998 CDS 100% 13.200 9.240 N HRH1 n/a
9 TRCN0000009063 GCTCTGGTTCTATGCCAAGAT pLKO.1 715 CDS 100% 4.950 3.465 N HRH1 n/a
10 TRCN0000271225 CCTCTGCTGGATCCCTTATTT pLKO_005 1372 CDS 100% 15.000 9.000 N HRH1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001098212.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06401 pDONR223 100% 99.9% 99.7% None 923T>A n/a
2 ccsbBroad304_06401 pLX_304 0% 99.9% 99.7% V5 923T>A n/a
3 TRCN0000476681 ACAGTAACAACTGCGATTCACGCA pLX_317 25.7% 99.9% 99.7% V5 923T>A n/a
4 TRCN0000489359 AATGTGCTTGCAATTCCCTGCTGT pLX_317 26.1% 99.9% 99.7% V5 (not translated due to prior stop codon) 1369G>A n/a
5 TRCN0000489376 AGAGGCTGACGGACTCCCGCAACT pLX_317 27.1% 99.8% 99.5% V5 1369G>A;1461_1462insG n/a
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