Construct: ORF TRCN0000476688
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007010.1_s317c1
- Derived from:
- ccsbBroadEn_13725
- DNA Barcode:
- CAAACAAGTCCCTCGTCTTCTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SNRPD2P2 (645339)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476688
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6633 | SNRPD2 | small nuclear ribonucleopro... | NM_001369751.1 | 38.4% | 30.7% | (many diffs) |
| 2 | human | 6633 | SNRPD2 | small nuclear ribonucleopro... | NM_177542.3 | 38.4% | 30.7% | (many diffs) |
| 3 | human | 6633 | SNRPD2 | small nuclear ribonucleopro... | NM_004597.6 | 35.3% | 28.3% | (many diffs) |
| 4 | human | 645339 | SNRPD2P2 | small nuclear ribonucleopro... | NR_033826.1 | 21.2% | 1_410del;576_776del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 218
- ORF end:
- 383
- ORF length:
- 165
- Sequence:
-
1 TTTTATGCGA TGGAGTTTCC CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG 61 CACTTGATGT AATTCTCCTT GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC 121 AAGCCTCAGA CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGCTAGCAT 181 CGATTGATCA ACAAGTTTGT ACAAAAAAGT TGGCACCATG GTGCTGGAGA ATGTGAAGGA 241 GATGTGTACT GAGGTCCCCA AGGGGGGCAA CGGTGGCAAG GGCAAGAAGA AGTCCAAGCC 301 AGCCAACAAA GACCACTTCA TCTCCAAGCT CTTCCTGTGC AGGGACTCAG TCATCACCAA 361 CAAGTGGGGG CCACCTCCCC ATTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 421 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 481 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA AACAAGTCCC 541 TCGTCTTCTT TAACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 601 tt