Construct: ORF TRCN0000476710
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015284.1_s317c1
- Derived from:
- ccsbBroadEn_03963
- DNA Barcode:
- GCACGTTTCGATCCGCGCTTTTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IPPK (64768)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476710
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64768 | IPPK | inositol-pentakisphosphate ... | NM_022755.6 | 100% | 100% | |
| 2 | human | 64768 | IPPK | inositol-pentakisphosphate ... | XM_017015041.1 | 80.8% | 81.6% | 1169_1244del;1329_1330ins220 |
| 3 | human | 64768 | IPPK | inositol-pentakisphosphate ... | XR_001746365.1 | 46.7% | 1_271del;1185_1186ins62;1683_2957del | |
| 4 | mouse | 75678 | Ippk | inositol 1,3,4,5,6-pentakis... | NM_199056.3 | 87.5% | 91% | (many diffs) |
| 5 | mouse | 75678 | Ippk | inositol 1,3,4,5,6-pentakis... | NM_001276399.1 | 78.3% | 79.4% | (many diffs) |
| 6 | mouse | 75678 | Ippk | inositol 1,3,4,5,6-pentakis... | XM_017315628.1 | 58% | 56.7% | (many diffs) |
| 7 | mouse | 75678 | Ippk | inositol 1,3,4,5,6-pentakis... | NR_075085.1 | 42.6% | (many diffs) | |
| 8 | mouse | 75678 | Ippk | inositol 1,3,4,5,6-pentakis... | XR_001780827.1 | 42.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1539
- ORF length:
- 1473
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agaggggaag atggacgaga atgaatgggg gtaccacgga gagggcaata 121 agagcctggt ggtggcccac gcgcagcgct gcgtcgtgct gcggtttctg aagtttcctc 181 caaataggaa gaagacctcg gaagagatat ttcaacacct gcagaacata gtggactttg 241 ggaaaaatgt catgaaggag tttttggggg agaactatgt tcattatggg gaggtcgttc 301 agctaccttt agagtttgtg aaacagcttt gtttaaagat acaatctgaa agaccagagt 361 ctcgctgtga caaggacctg gatactctca gtggttacgc tatgtgcctt cctaatttaa 421 ccagactcca aacctaccgc tttgcagagc accggccgat tctgtgtgta gagattaagc 481 caaaatgtgg gtttattcct ttctcgagtg atgtcacgca tgagatgaag cataaggtct 541 gtcgatactg catgcaccag cacctcaagg tagcaactgg gaagtggaag cagatcagca 601 aatactgtcc ccttgatctc tactcaggaa acaaacagag aatgcacttt gccttgaaga 661 gtttgctgca ggaggcacag aacaacttga agatatttaa gaatggtgag ctgatttacg 721 gctgcaaaga tgcccggagc cccgtggctg actggagcga gcttgcacac cacctgaagc 781 cgttcttctt cccttccaac ggcctggcca gtgggcccca ctgcacaagg gctgtgatca 841 gggagctggt gcacgtgatc acacgggtgc tgctgagtgg ctcggacaag ggccgggcag 901 gcaccctgag tccggggctc gggcctcagg gcccgcgagt ctgcgaagcc agccctttca 961 gtaggagcct tcgctgccaa ggaaaaaaca ccccagagcg ctcggggtta ccgaagggct 1021 gtcttctgta caaaaccctc caggtgcaga tgttggacct gctggacatc gaaggcctct 1081 accctctgta caaccgggtt gagcgatacc tggaagagtt tcccgaggag agaaaaacct 1141 tacaaataga tgggccttat gatgaagcat tttaccagaa gctgcttgac ctttccactg 1201 aggatgacgg gacagtggcc ttcgcgctaa cgaaggtgca gcagtaccgc gtcgccatga 1261 ctgccaagga ctgctCCATC ATGATTGCAC TGTCTCCCTG TCTGCAGGAT GCCAGCTCTG 1321 ATCAAAGGCC TGTCGTCCCT TCATCGAGGT CCAGGTTTGC CTTTTCCGTG TCTGTGCTGG 1381 ACCTTGACCT CAAGCCCTAC GAGAGCATTC CCCATCAGTA TAAACTGGAC GGCAAGATCG 1441 TCAACTATTA TTCAAAGACT GTACGTGCCA AAGACAACGC CGTGATGTCG ACTCGGTTCA 1501 AGGAAAGCGA AGATTGCACA TTAGTTCTCC ACAAGGTCTA CCCAACTTTC TTGTACAAAG 1561 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1621 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1681 ACGAGCACGT TTCGATCCGC GCTTTTAAAC GCGTTAAGTC gacaatcaac ctctggatta 1741 caaaatttgt gaaagatt